{"id":"5da56186-d6c9-5e83-baf7-7fe4651795f5","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-knockdown-export","predicate":"silencing_reduces","statement":"Silencing CRAT in primary human myotubes reduced acetylcarnitine export by 47%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"69d6c2c6-1d2c-58e4-a9bf-b0d0fcfd31a4","mechanism_event_label":"An independent enzyme controls acetyl-group export from muscle cells.","subject":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"object":{"id":"87181d27-cc7e-5f1f-8f14-4943841414c3","slug":"human-myotube-acetylcarnitine-efflux","display_name":"Human primary myotube acetylcarnitine export","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"69d6c2c6-1d2c-58e4-a9bf-b0d0fcfd31a4","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-crat-knockdown-export-event","event_type":"observed_relationship","label":"An independent enzyme controls acetyl-group export from muscle cells.","description":"Silencing CRAT in primary human myotubes reduced acetylcarnitine export by 47%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"507b3d2c-db75-51d1-823a-0677e0c1a0e8","slug":"human-crat","display_name":"Human carnitine acetyltransferase / CRAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"87181d27-cc7e-5f1f-8f14-4943841414c3","slug":"human-myotube-acetylcarnitine-efflux","display_name":"Human primary myotube acetylcarnitine export","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text Figure 6 and methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human myotubes; adenoviral silencing, approximately 60% lower enzyme activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell culture, not human supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An independent enzyme controls acetyl-group export from muscle cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Muscle-specific deletion of carnitine acetyltransferase compromises glucose tolerance and metabolic flexibility. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560225/ · DOI 10.1016/j.cmet.2012.04.005","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d8b23be1-cbe4-502d-876d-08a1652faf6e","evidence_kind":"source_excerpt","locator":"Lines 170-176","start_line":170,"end_line":176,"excerpt":"## l-carnitine-crat-knockdown-export\nAn independent enzyme controls acetyl-group export from muscle cells.\nSilencing CRAT in primary human myotubes reduced acetylcarnitine export by 47%.\nModel: Human myotubes; adenoviral silencing, approximately 60% lower enzyme activity.\nLimitations: Cell culture, not human supplementation.\nEvidence access: Primary full-text Figure 6 and methods\nMuscle-specific deletion of carnitine acetyltransferase compromises glucose tolerance and metabolic flexibility. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560225/ · DOI 10.1016/j.cmet.2012.04.005","model_system":"Human myotubes; adenoviral silencing, approximately 60% lower enzyme activity.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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