{"id":"5d6a4a25-1967-5b7b-98b5-722e9e61ca82","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-nadh","predicate":"supports_oxidation","statement":"LPO/H2O2/iodide oxidized NADH to a product chemically distinct from NAD+, unlike the thiocyanate and bromide systems.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"123949ad-fc26-532c-a998-9b22610f93bd","mechanism_event_label":"The NADH reaction did not simply regenerate normal NAD+.","subject":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"object":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"123949ad-fc26-532c-a998-9b22610f93bd","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-nadh-event","event_type":"observed_relationship","label":"The NADH reaction did not simply regenerate normal NAD+.","description":"LPO/H2O2/iodide oxidized NADH to a product chemically distinct from NAD+, unlike the thiocyanate and bromide systems.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3d671567-4fb6-5b5f-a328-156987d5b396","slug":"lactoperoxidase-family","display_name":"Lactoperoxidase enzyme family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Cell-free LPO/H2O2 reactions with nicotinamide nucleotides.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_category","value_text":"Study-specific exposure, including pharmacological and in-vitro conditions; normal is the schema fallback outside the three availability categories.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Chemical oxidation is not evidence that supplementation drains niacin in vivo; the authors expected thiocyanate oxidation to predominate in milk/saliva.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium iodide research collection; shared-anion and comparator studies are not all KI interventions.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium-iodide","display_name":"Potassium iodide","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The NADH reaction did not simply regenerate normal NAD+.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The oxidation of reduced nicotinamide nucleotides by hydrogen peroside in the presence of lactoperoxidase and thiocyanate, iodide or bromide. · 1970 · https://pubmed.ncbi.nlm.nih.gov/4317722/ · DOI 10.1042/bj1170791","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c7fcba5f-8055-5618-a278-04481fc42fd4","evidence_kind":"source_excerpt","locator":"Lines 256-262","start_line":256,"end_line":262,"excerpt":"## ki-nadh\nThe NADH reaction did not simply regenerate normal NAD+.\nLPO/H2O2/iodide oxidized NADH to a product chemically distinct from NAD+, unlike the thiocyanate and bromide systems.\nModel: Cell-free LPO/H2O2 reactions with nicotinamide nucleotides.\nLimitations: Chemical oxidation is not evidence that supplementation drains niacin in vivo; the authors expected thiocyanate oxidation to predominate in milk/saliva.\nEvidence location: Primary abstract\nThe oxidation of reduced nicotinamide nucleotides by hydrogen peroside in the presence of lactoperoxidase and thiocyanate, iodide or bromide. · 1970 · https://pubmed.ncbi.nlm.nih.gov/4317722/ · DOI 10.1042/bj1170791","model_system":"Cell-free LPO/H2O2 reactions with nicotinamide nucleotides.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curation paraphrase; primary evidence location and source kind retained above.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc3ce411-f68f-5eb8-87c1-1592665f9cb6","stable_key":"import-79018983-9179-5c1a-8e7a-d6f47a13582b","title":"Potassium iodide: thyroid and non-thyroid mechanisms, interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-study references, chemical references and label statements individually identified. Not publisher full text.","file_path":"","sha256":"3ff9da818dd7a763a59c6abcff2eaee136d7f887a777034feaa5ba304a8fa6ce","revision_id":"53f2ae88-8a91-5326-ac49-3bb510ca78c3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}