{"id":"5d37db6b-0ede-5b78-8ec4-a2a8187fcd71","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-stat3-rescue","predicate":"when_overexpressed_reduces_tested","statement":"STAT3 overexpression reversed tested cucurbitacin B-associated lipid ROS and iron changes, whereas STAT3 silencing enhanced them.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f92b71ea-089a-5792-9a3a-f16cfa3ee9a9","mechanism_event_label":"Changing a signaling protein altered the iron-dependent response.","subject":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"object":{"id":"cec3de0d-ba4c-55ab-863c-1b16e4c38e61","slug":"human-cucurbitacin-lipid-peroxidation","display_name":"Human cancer-cell lipid peroxidation during cucurbitacin B exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f92b71ea-089a-5792-9a3a-f16cfa3ee9a9","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-stat3-rescue-event","event_type":"observed_relationship","label":"Changing a signaling protein altered the iron-dependent response.","description":"STAT3 overexpression reversed tested cucurbitacin B-associated lipid ROS and iron changes, whereas STAT3 silencing enhanced them.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dc129d3d-aaa0-5511-9a60-3e3880b0eb0f","slug":"stat3","display_name":"STAT3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cec3de0d-ba4c-55ab-863c-1b16e4c38e61","slug":"human-cucurbitacin-lipid-peroxidation","display_name":"Human cancer-cell lipid peroxidation during cucurbitacin B exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"59d6d1cd-df32-5b58-b950-3188bc7b95d6","slug":"iron-ii","display_name":"Ferrous iron","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"cec30c19-2d02-5f08-9a38-a0fa2b49e22d","slug":"human-cucurbitacin-ferroptosis","display_name":"Human NSCLC ferroptotic response during cucurbitacin B exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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