{"id":"5ce8ad50-9339-51a9-99ef-94047a7d9b2c","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-analytical-interference","predicate":"causes_assay_interference","statement":"N-acetyl-SAC decomposed in the detector source and interfered with SAC quantification unless chromatographic separation was used.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"01bd7e02-e8cf-59ec-b66f-bc3413ea89a2","mechanism_event_label":"A metabolite can make an analytical SAC signal misleading.","subject":{"id":"0837bab7-ef2d-533f-9025-740a1c76cdf3","slug":"n-acetyl-s-allylcysteine","display_name":"N-acetyl-S-allyl-L-cysteine","entity_type_key":"small_molecule"},"object":{"id":"3cb7da88-0d29-5975-a7b5-10b205bc8531","slug":"sac-lcms-source-interference","display_name":"SAC/NAc-SAC mass-spectrometric source interference","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"01bd7e02-e8cf-59ec-b66f-bc3413ea89a2","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-analytical-interference-event","event_type":"observed_relationship","label":"A metabolite can make an analytical SAC signal misleading.","description":"N-acetyl-SAC decomposed in the detector source and interfered with SAC quantification unless chromatographic separation was used.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0837bab7-ef2d-533f-9025-740a1c76cdf3","slug":"n-acetyl-s-allylcysteine","display_name":"N-acetyl-S-allyl-L-cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3cb7da88-0d29-5975-a7b5-10b205bc8531","slug":"sac-lcms-source-interference","display_name":"SAC/NAc-SAC mass-spectrometric source interference","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"n-acetyl-s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Calibration and source-fragmentation experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Analytical assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"SAC and metabolite standards with chromatographic separation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"LC-MS/MS assay validation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Analytical artifact, not biological conversion or an increase in in-vivo SAC.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat plasma analytical matrix","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A metabolite can make an analytical SAC signal misleading.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[28561204] Oral Administration of (S)-Allyl-l-Cysteine and Aged Garlic Extract to Rats: Determination of Metabolites and Their Pharmacokinetics. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28561204/ · DOI 10.1055/s-0043-111895","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Ex vivo","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"LC-MS/MS assay validation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fb0b063d-ff85-5873-846e-5be05f2a7c7b","evidence_kind":"source_excerpt","locator":"Lines 50-57","start_line":50,"end_line":57,"excerpt":"## s-allylcysteine-analytical-interference\nA metabolite can make an analytical SAC signal misleading.\nN-acetyl-SAC decomposed in the detector source and interfered with SAC quantification unless chromatographic separation was used.\nModel: LC-MS/MS assay validation\nLimitations: Analytical artifact, not biological conversion or an increase in in-vivo SAC.\nEvidence access: Primary abstract\n[28561204] Oral Administration of (S)-Allyl-l-Cysteine and Aged Garlic Extract to Rats: Determination of Metabolites and Their Pharmacokinetics. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28561204/ · DOI 10.1055/s-0043-111895\nStructured context: {\"organism\": \"Rat plasma analytical matrix\", \"tissue_or_cell_type\": \"LC-MS/MS assay validation\", \"dose\": \"Calibration and source-fragmentation experiment\", \"duration\": \"Analytical assay\", \"route\": \"Ex vivo\", \"experimental_comparison\": \"SAC and metabolite standards with chromatographic separation\", \"acting_entity\": \"n-acetyl-s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"LC-MS/MS assay validation","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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