{"id":"5ccebc5c-b05e-5364-84b2-636d00eb8c05","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-selenium-receptor","predicate":"low_availability_decreases","statement":"Low-selenium culture conditions were associated with defective IP3R palmitoylation and reduced receptor expression in the SELENOK study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"ea1fbb4d-f044-57a2-9107-8430f30ed6c4","mechanism_event_label":"The cell may still make the message but lose a properly maintained receptor.","subject":{"id":"dc89d7e2-8f89-577b-838f-42bf5d8ec7c8","slug":"selenium","display_name":"Selenium","entity_type_key":"nutrient_element"},"object":{"id":"960e97ec-b639-534d-8cd5-ece8d8ac1d06","slug":"ip3r-palmitoylation","display_name":"IP3 receptor palmitoylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ea1fbb4d-f044-57a2-9107-8430f30ed6c4","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-selenium-receptor-event","event_type":"biochemical_relationship","label":"The cell may still make the message but lose a properly maintained receptor.","description":"Low-selenium culture conditions were associated with defective IP3R palmitoylation and reduced receptor expression in the SELENOK study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83ea17c8-4611-596b-9be8-1a0df1c9b42e","slug":"selenok","display_name":"SELENOK","entity_type_key":"protein"},"role":"dependent_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a34badca-ef8a-560f-a8f1-04870be92cbb","slug":"ip3r-abundance","display_name":"IP3 receptor protein abundance","entity_type_key":"cellular_process"},"role":"downstream_outcome","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dc89d7e2-8f89-577b-838f-42bf5d8ec7c8","slug":"selenium","display_name":"Selenium","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"960e97ec-b639-534d-8cd5-ece8d8ac1d06","slug":"ip3r-palmitoylation","display_name":"IP3 receptor palmitoylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/25368151.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\", \"start_char\": 0, \"end_char\": 1873, \"text_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Immune-cell perturbation, co-immunoprecipitation and palmitoylation assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Selk deletion, low-selenium media, DHHC6 knockdown and IP3R cysteine mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Selenium limitation and genetic deletion are distinct. This does not establish that inositol supplements repair selenium-dependent receptor failure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mouse immune cells and mammalian cell models","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The cell may still make the message but lose a properly maintained receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. (2014). https://pubmed.ncbi.nlm.nih.gov/25368151/ DOI: 10.1073/pnas.1417176111","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"ER-associated IP3 receptor machinery","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"8d49ffd3-79f8-51bc-9cf8-b7d38b5cfa36","evidence_kind":"source_excerpt","locator":"Lines 1068-1079","start_line":1068,"end_line":1079,"excerpt":"### ino-selenium-receptor\nLow-selenium culture conditions were associated with defective IP3R palmitoylation and reduced receptor expression in the SELENOK study.\nCondition category: nutrient_deficiency\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The cell may still make the message but lose a properly maintained receptor.\norganism: Mouse immune cells and mammalian cell models\ntissue_or_cell_type: ER-associated IP3 receptor machinery\nexperimental_model: Immune-cell perturbation, co-immunoprecipitation and palmitoylation assays\nlimitations: Selenium limitation and genetic deletion are distinct. This does not establish that inositol supplements repair selenium-dependent receptor failure.\nexposure: Selk deletion, low-selenium media, DHHC6 knockdown and IP3R cysteine mutants\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/25368151.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\", \"start_char\": 0, \"end_char\": 1873, \"text_sha256\": \"84452ee9a1f04bf54aba8030e5384d46a1dafdbb460281fd99c244e57d6644d9\"}\n[ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. (2014). https://pubmed.ncbi.nlm.nih.gov/25368151/ DOI: 10.1073/pnas.1417176111","model_system":"Immune-cell perturbation, co-immunoprecipitation and palmitoylation assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p25368151] Stable expression and function of the inositol 1,4,5-triphosphate receptor requires palmitoylation by a DHHC6/selenoprotein K complex. (2014). https://pubmed.ncbi.nlm.nih.gov/25368151/ DOI: 10.1073/pnas.1417176111","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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