{"id":"5cb668af-b637-5c17-b08f-c9fb5bb0f410","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-no-depolymerization-effect","predicate":"does_not_change_tested","statement":"In the 2010 purified-actin assays, cucurbitacin I did not change depolymerization rate at concentrations active in cells; high concentrations delayed polymerization initiation without changing its subsequent rate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"4fd39d39-dab4-5638-ba3e-c22b7f247224","mechanism_event_label":"A dramatic cell phenotype was not reproduced by every purified-actin endpoint.","subject":{"id":"55ef0ba6-495d-5cc4-88c5-0772ab25d0fe","slug":"cucurbitacin-i","display_name":"Cucurbitacin I","entity_type_key":"small_molecule"},"object":{"id":"24579623-993e-5330-801b-b438cf595a0b","slug":"purified-cucurbitacin-f-actin","display_name":"Purified F-actin in cucurbitacin assays, species as reported","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4fd39d39-dab4-5638-ba3e-c22b7f247224","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-i-no-depolymerization-effect-event","event_type":"observed_relationship","label":"A dramatic cell phenotype was not reproduced by every purified-actin endpoint.","description":"In the 2010 purified-actin assays, cucurbitacin I did not change depolymerization rate at concentrations active in cells; 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not an automatic scientific contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A dramatic cell phenotype was not reproduced by every purified-actin endpoint.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21124831/ · DOI 10.1371/journal.pone.0014039","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"dd6d5519-95a9-5e13-b352-b3ec6c1b2225","evidence_kind":"source_excerpt","locator":"Lines 76-82","start_line":76,"end_line":82,"excerpt":"## cucurbitacin-i-no-depolymerization-effect\nA dramatic cell phenotype was not reproduced by every purified-actin endpoint.\nIn the 2010 purified-actin assays, cucurbitacin I did not change depolymerization rate at concentrations active in cells; high concentrations delayed polymerization initiation without changing its subsequent rate.\nModel: Purified-actin experiments alongside canine and mouse cell models.\nLimitations: Different member, concentration and endpoint from cucurbitacin E stabilization; not an automatic scientific contradiction.\nEvidence access: Primary full text PMC2991314\nCucurbitacin I inhibits cell motility by indirectly interfering with actin dynamics. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21124831/ · DOI 10.1371/journal.pone.0014039","model_system":"Purified-actin experiments alongside canine and mouse cell models.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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