{"id":"5c902ffb-6b17-5f79-9773-c3033fdd7239","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-potassium-gate","predicate":"high_extracellular_level_blocks_release","statement":"High extracellular potassium blocked silica-induced caspase-1 processing and IL-1 beta release, including during low-dose uptake blockade.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1f2adccb-23e4-5e94-abbc-f18d83becf8a","mechanism_event_label":"The potassium gradient gates this inflammatory response.","subject":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"object":{"id":"c17455e2-5805-55e7-9ecc-09c953115c84","slug":"mouse-il1b","display_name":"Mouse IL-1 beta","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"1f2adccb-23e4-5e94-abbc-f18d83becf8a","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-potassium-gate-event","event_type":"observed_relationship","label":"The potassium gradient gates this inflammatory response.","description":"High extracellular potassium blocked silica-induced caspase-1 processing and IL-1 beta release, including during low-dose uptake blockade.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c17455e2-5805-55e7-9ecc-09c953115c84","slug":"mouse-il1b","display_name":"Mouse IL-1 beta","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b2d4231b-3893-5d4e-8b52-d0effdaa26c9","slug":"crystalline-silica","display_name":"Crystalline silica / quartz particles","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ae25f14e-2a54-5094-9952-a97b718f94a3","slug":"mouse-nlrp3","display_name":"Mouse NLRP3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"69b5a136-93e3-567b-a97a-d33853229c81","slug":"mouse-casp1","display_name":"Mouse caspase-1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse macrophages; 130 mM KCl experimental condition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a potassium supplement treatment; no specific channel identity was established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The potassium gradient gates this inflammatory response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Activation of NLRP3 inflammasome by crystalline structures via cell surface contact. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25445147/ · DOI 10.1038/srep07281","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5dec40a5-2fd8-5145-96e8-2ab40db7bb7a","evidence_kind":"source_excerpt","locator":"Lines 480-486","start_line":480,"end_line":486,"excerpt":"## silica-potassium-gate\nThe potassium gradient gates this inflammatory response.\nHigh extracellular potassium blocked silica-induced caspase-1 processing and IL-1 beta release, including during low-dose uptake blockade.\nModel: Mouse macrophages; 130 mM KCl experimental condition.\nLimitations: Not a potassium supplement treatment; no specific channel identity was established.\nEvidence access: Primary full text\nActivation of NLRP3 inflammasome by crystalline structures via cell surface contact. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25445147/ · DOI 10.1038/srep07281","model_system":"Mouse macrophages; 130 mM KCl experimental condition.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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