{"id":"5c7bfc9a-c617-59b7-b285-91654d70e0d5","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-trpv1-qx314","predicate":"enables_prolonged_block_by","statement":"Eugenol activated inward current in capsaicin-sensitive trigeminal neurons and, with QX-314, produced sodium-channel and action-potential block that persisted after washout.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"601ce104-4c7c-5235-8b66-a13afa2e949f","mechanism_event_label":"Eugenol activated inward current in capsaicin-sensitive trigeminal neurons and, with QX-314, produced sodium-channel and action-potential block that persisted after washout.","subject":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"object":{"id":"d1c2cd56-cc59-5b17-9ef1-aded5d22f963","slug":"qx314","display_name":"QX-314 permanently charged sodium-channel blocker","entity_type_key":"drug"},"evidence_count":1,"mechanism_event":{"id":"601ce104-4c7c-5235-8b66-a13afa2e949f","stable_key":"2f309d3a-d252-5dfb-ae24-993a625f1b6c:eugenol-trpv1-qx314-event","event_type":"observed_relationship","label":"Eugenol activated inward current in capsaicin-sensitive trigeminal neurons and, with QX-314, produced sodium-channel and action-potential block that persisted after washout.","description":"Eugenol activated inward current in capsaicin-sensitive trigeminal neurons and, with QX-314, produced sodium-channel and action-potential block that persisted after washout.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4bea4ede-273a-5d8f-a4e7-e928156d2d82","slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d1c2cd56-cc59-5b17-9ef1-aded5d22f963","slug":"qx314","display_name":"QX-314 permanently charged sodium-channel blocker","entity_type_key":"drug"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"97ef6387-149c-5a0a-8adc-15725324f62e","slug":"voltage-gated-sodium-channels","display_name":"Voltage-gated sodium channels","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Eugenol alone or with QX-314","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute plus washout","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rodent trigeminal-ganglion nociceptive neurons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Persistent cellular block is not a validated human dental regimen and may involve safety risks not tested here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Eugenol chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"eugenol","display_name":"Eugenol","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rodent trigeminal-ganglion nociceptive neurons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Eugenol activated inward current in capsaicin-sensitive trigeminal neurons and, with QX-314, produced sodium-channel and action-potential block that persisted after washout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Co-Application of Eugenol and QX-314 Elicits the Prolonged Blockade of Voltage-Gated Sodium Channels in Nociceptive Trigeminal Ganglion Neurons. (2020). https://pubmed.ncbi.nlm.nih.gov/33167484/ DOI: 10.3390/biom10111513","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro co-application","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"TRPV1-associated entry, sodium current and action potentials","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3ca89ead-40a6-5c4e-b93c-581fa8f2bcbe","evidence_kind":"source_excerpt","locator":"Lines 66-75","start_line":66,"end_line":75,"excerpt":"## eugenol-trpv1-qx314\nEugenol activated inward current in capsaicin-sensitive trigeminal neurons and, with QX-314, produced sodium-channel and action-potential block that persisted after washout.\nModel/species: Rodent trigeminal-ganglion nociceptive neurons\nTissue/system: TRPV1-associated entry, sodium current and action potentials\nExposure: Eugenol alone or with QX-314\nRoute: In vitro co-application\nDuration: Acute plus washout\nLimits: Persistent cellular block is not a validated human dental regimen and may involve safety risks not tested here.\nPrimary reference: Co-Application of Eugenol and QX-314 Elicits the Prolonged Blockade of Voltage-Gated Sodium Channels in Nociceptive Trigeminal Ganglion Neurons. (2020). https://pubmed.ncbi.nlm.nih.gov/33167484/ DOI: 10.3390/biom10111513\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Rodent trigeminal-ganglion nociceptive neurons","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f2ff5a4-7264-57fc-8368-46b9768ec668","stable_key":"import-2f309d3a-d252-5dfb-ae24-993a625f1b6c","title":"Eugenol: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"28c421c41a2fbfb00f3036084d43389247cfbfbad8f7549b7529fb4f0e84f61d","revision_id":"3377bcaa-7d21-522a-8571-50ff1977aba0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}