{"id":"5c745d45-2bf9-54f3-a8d5-52b9fb7ddab8","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nicotinic-rat","predicate":"inhibits","statement":"Agmatine at 10 micromolar substantially blocked DMPP depolarization and synaptic transmission in rat superior cervical ganglion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"8e0e90b0-c308-5af0-9ca1-6ab5ee1308ff","mechanism_event_label":"A second tissue showed nicotinic inhibition at a different exposure.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"418b8495-6b99-559d-80ab-1a8dfa0c8b70","slug":"rat-superior-cervical-ganglion-nicotinic-transmission","display_name":"Rat superior cervical ganglion nicotinic transmission","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8e0e90b0-c308-5af0-9ca1-6ab5ee1308ff","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nicotinic-rat-event","event_type":"observed_relationship","label":"A second tissue showed nicotinic inhibition at a different exposure.","description":"Agmatine at 10 micromolar substantially blocked DMPP depolarization and synaptic transmission in rat superior cervical ganglion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"418b8495-6b99-559d-80ab-1a8dfa0c8b70","slug":"rat-superior-cervical-ganglion-nicotinic-transmission","display_name":"Rat superior cervical ganglion nicotinic transmission","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat isolated ganglion experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different tissue sensitivities are not interchangeable dose estimates.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second tissue showed nicotinic inhibition at a different exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine acts as an antagonist of neuronal nicotinic receptors. · 1990 · https://pubmed.ncbi.nlm.nih.gov/2331571/ · DOI 10.1111/j.1476-5381.1990.tb14680.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d0b692b1-dccc-522c-87ff-3f4db17d6283","evidence_kind":"source_excerpt","locator":"Lines 332-338","start_line":332,"end_line":338,"excerpt":"## agmatine-sulfate-nicotinic-rat\nA second tissue showed nicotinic inhibition at a different exposure.\nAgmatine at 10 micromolar substantially blocked DMPP depolarization and synaptic transmission in rat superior cervical ganglion.\nModel: Rat isolated ganglion experiments.\nLimitations: Different tissue sensitivities are not interchangeable dose estimates.\nEvidence access: Primary abstract\nAgmatine acts as an antagonist of neuronal nicotinic receptors. · 1990 · https://pubmed.ncbi.nlm.nih.gov/2331571/ · DOI 10.1111/j.1476-5381.1990.tb14680.x","model_system":"Rat isolated ganglion experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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