{"id":"5c55cecd-5ba6-574e-a28d-a6d5d43fe509","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-hmgcr-binding","predicate":"competitively_inhibits_tested","statement":"Monacolin K acid sodium salt competitively inhibited rat liver HMG-CoA reductase with respect to HMG-CoA; the reported Ki was 0.49 nM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"0b5b0f80-531b-5575-9f4d-5eaf89242e10","mechanism_event_label":"The active preparation competed with the enzyme substrate.","subject":{"id":"25463ec8-d3f0-5924-bc21-3eec7f66f2c8","slug":"lovastatin-acid-sodium","display_name":"Monacolin K hydroxy-acid sodium salt","entity_type_key":"small_molecule"},"object":{"id":"49a9fd79-4ebe-5c77-ad3a-a026d354b753","slug":"rat-hmgcr","display_name":"Rat HMG-CoA reductase / Hmgcr","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0b5b0f80-531b-5575-9f4d-5eaf89242e10","stable_key":"4910e17b-aaec-52cd-b9e5-caca85d817ee:red-yeast-rice-hmgcr-binding-event","event_type":"observed_relationship","label":"The active preparation competed with the enzyme substrate.","description":"Monacolin K acid sodium salt competitively inhibited rat liver HMG-CoA reductase with respect to HMG-CoA; the reported Ki was 0.49 nM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"25463ec8-d3f0-5924-bc21-3eec7f66f2c8","slug":"lovastatin-acid-sodium","display_name":"Monacolin K hydroxy-acid sodium salt","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49a9fd79-4ebe-5c77-ad3a-a026d354b753","slug":"rat-hmgcr","display_name":"Rat HMG-CoA reductase / Hmgcr","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"81e5bb47-0c11-54c9-a0ec-5c213930c173","slug":"hmg-coa","display_name":"(S)-3-Hydroxy-3-methylglutaryl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3df322fd-ac21-5bd1-aa46-4af1ec708b57","slug":"mevalonate","display_name":"Mevalonate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary PDF, p335 and Figure 3 visually inspected","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Partially purified rat liver microsomal reductase; radiolabeled product assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Assay-specific inhibition constant, not a human plasma threshold or potency of an entire rice product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Red yeast rice collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"red-yeast-rice","display_name":"Red yeast rice","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The active preparation competed with the enzyme substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[7380744] Monacolin K, a new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase. · 1980 · https://pubmed.ncbi.nlm.nih.gov/7380744/ · DOI 10.7164/antibiotics.33.334","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8f561f6f-bd21-5e05-9a78-7a3ca7765526","evidence_kind":"source_excerpt","locator":"Lines 52-58","start_line":52,"end_line":58,"excerpt":"## red-yeast-rice-hmgcr-binding\nThe active preparation competed with the enzyme substrate.\nMonacolin K acid sodium salt competitively inhibited rat liver HMG-CoA reductase with respect to HMG-CoA; the reported Ki was 0.49 nM.\nModel: Partially purified rat liver microsomal reductase; radiolabeled product assay.\nLimitations: Assay-specific inhibition constant, not a human plasma threshold or potency of an entire rice product.\nEvidence access: Primary PDF, p335 and Figure 3 visually inspected\n[7380744] Monacolin K, a new hypocholesterolemic agent that specifically inhibits 3-hydroxy-3-methylglutaryl coenzyme A reductase. · 1980 · https://pubmed.ncbi.nlm.nih.gov/7380744/ · DOI 10.7164/antibiotics.33.334","model_system":"Partially purified rat liver microsomal reductase; radiolabeled product assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"261a12bc-3d00-52a4-a5ec-70e174390596","stable_key":"import-4910e17b-aaec-52cd-b9e5-caca85d817ee","title":"Red yeast rice: constituents, mevalonate, CoQ and product-specific interactions (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"271bbcecaaacc76ad4d06718ae592dc6a1b33e3e459adfdd36cbd0ac5f09c0f6","revision_id":"9c30956c-206c-5c49-ae42-81b070088e26","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}