{"id":"5c52d514-f43c-5d14-bcc0-fdee4854ca68","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-ass1-energy","predicate":"powers","statement":"The ASS1 reaction uses ATP cleavage to AMP and pyrophosphate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"02899f90-0627-5b40-9f10-554c034da2ac","mechanism_event_label":"Arginine regeneration has an energy cost.","subject":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"object":{"id":"d46fbb06-9880-57cf-a7eb-1238648cdbb4","slug":"ass1","display_name":"Human argininosuccinate synthetase 1 / ASS1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"02899f90-0627-5b40-9f10-554c034da2ac","stable_key":"a9828b14-fbd7-57bf-9e01-0d52d1b42a1f:citrulline-ass1-energy-event","event_type":"biochemical_relationship","label":"Arginine regeneration has an energy cost.","description":"The ASS1 reaction uses ATP cleavage to AMP and pyrophosphate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f53c7bfb-3b91-56fe-bebd-28623cfe13a0","slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0a0923d3-72b7-5d6a-bf3a-5a7a3071a09b","slug":"l-aspartate","display_name":"L-Aspartate","entity_type_key":"small_molecule"},"role":"cosubstrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"energy_substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d4847103-f17d-57bf-8ad9-04436b9eb16f","slug":"argininosuccinate","display_name":"L-Argininosuccinate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d46fbb06-9880-57cf-a7eb-1238648cdbb4","slug":"ass1","display_name":"Human argininosuccinate synthetase 1 / ASS1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/citrulline-research/18323623.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"079be80a0ea1a47308efb069d3c9522c96c0aa5923ad1d7ce79ac620b2d9502f\", \"start_char\": 0, \"end_char\": 840, \"text_sha256\": \"079be80a0ea1a47308efb069d3c9522c96c0aa5923ad1d7ce79ac620b2d9502f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystal structure and biochemical reaction characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Citrulline and aspartate bound to recombinant enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure and established reaction; no clinical magnesium, aspartate or ATP deficiency threshold is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Citrulline research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"citrulline","display_name":"L-Citrulline","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human ASS1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Arginine regeneration has an energy cost.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[citrulline-p18323623] Structure of human argininosuccinate synthetase. (2008). https://pubmed.ncbi.nlm.nih.gov/18323623/ DOI: 10.1107/s0907444907067455","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cytosolic arginine-regeneration reaction","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e077cca4-8790-5481-99c0-1ddb9e00a7a3","evidence_kind":"source_excerpt","locator":"Lines 151-162","start_line":151,"end_line":162,"excerpt":"### citrulline-ass1-energy\nThe ASS1 reaction uses ATP cleavage to AMP and pyrophosphate.\nCondition category: normal\nnutrient_topic: Citrulline research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Arginine regeneration has an energy cost.\norganism: Human ASS1\ntissue_or_cell_type: Cytosolic arginine-regeneration reaction\nexperimental_model: Crystal structure and biochemical reaction characterization\nlimitations: Structure and established reaction; no clinical magnesium, aspartate or ATP deficiency threshold is established.\nexposure: Citrulline and aspartate bound to recombinant enzyme\nevidence_span: {\"source_cache\": \"artifacts/citrulline-research/18323623.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"079be80a0ea1a47308efb069d3c9522c96c0aa5923ad1d7ce79ac620b2d9502f\", \"start_char\": 0, \"end_char\": 840, \"text_sha256\": \"079be80a0ea1a47308efb069d3c9522c96c0aa5923ad1d7ce79ac620b2d9502f\"}\n[citrulline-p18323623] Structure of human argininosuccinate synthetase. (2008). https://pubmed.ncbi.nlm.nih.gov/18323623/ DOI: 10.1107/s0907444907067455","model_system":"Crystal structure and biochemical reaction characterization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [citrulline-p18323623] Structure of human argininosuccinate synthetase. (2008). https://pubmed.ncbi.nlm.nih.gov/18323623/ DOI: 10.1107/s0907444907067455","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"112fd375-aa2a-59d2-8afd-6b2849bb79ee","stable_key":"import-a9828b14-fbd7-57bf-9e01-0d52d1b42a1f","title":"Citrulline: arginine recycling, nitrogen disposal and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"3720442dbabc28cacefd2145c7c97bfb937df26dca8527fde219ecd3735ed558","revision_id":"41ac8493-b004-59d7-99b3-8a3ca7163d3a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}