{"id":"5c3c1348-68a2-596a-8f89-1ce326789c9b","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:purified-mateos-fmd","predicate":"increases_in_recorded_experiment","statement":"Purified anthocyanins improved FMD in healthy humans in the 2019 experimental program.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0e8a275d-8942-522b-bff7-1106f2a9b107","mechanism_event_label":"Purified anthocyanins improved FMD in healthy humans in the 2019 experimental program.","subject":{"id":"ff2918ef-0592-5ffa-89d8-bee92100d783","slug":"purified-anthocyanins-mateos-2019","display_name":"Purified anthocyanin preparation in Rodriguez-Mateos 2019","entity_type_key":"chemical_species"},"object":{"id":"a557fb69-ee27-5be9-8318-227c570267ff","slug":"flow-mediated-dilation","display_name":"Brachial artery flow-mediated dilation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0e8a275d-8942-522b-bff7-1106f2a9b107","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:purified-mateos-fmd-event","event_type":"experimental_observation","label":"Purified anthocyanins improved FMD in healthy humans in the 2019 experimental program.","description":"**Metabolite-mediated vascular activity has been tested.** A 2019 program compared purified anthocyanins, blueberries and control preparations in people, related circulating metabolites to FMD, and tested selected metabolite administration in mice. Purified anthocyanins improved FMD, while fiber/mineral/vitamin control drinks did not show the same effect. Human associations, mouse interventions and blood-cell gene-expression changes are different evidence layers. They support further metabolite research; they do not prove every correlated molecule is necessary or sufficient in humans. [Rodriguez-Mateos et al., 2019](https://pubmed.ncbi.nlm.nih.gov/30772905/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ff2918ef-0592-5ffa-89d8-bee92100d783","slug":"purified-anthocyanins-mateos-2019","display_name":"Purified anthocyanin preparation in Rodriguez-Mateos 2019","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"Purified anthocyanin preparation","sequence_order":0,"notes":""},{"entity":{"id":"a557fb69-ee27-5be9-8318-227c570267ff","slug":"flow-mediated-dilation","display_name":"Brachial artery flow-mediated dilation","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"a1b1c9c3-cafe-5dd6-a590-f56222d3a4cc","slug":"anthocyanins","display_name":"Anthocyanins","entity_type_key":"chemical_species"},"role":"class represented in the tested mixture","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, figures, exact doses or endpoint-specific species assignments may remain unextracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Purified anthocyanin preparation\", \"comparator\": \"Control preparation\", \"endpoint\": \"Purified anthocyanins improved FMD in healthy humans in the 2019 experimental program.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Human purified-anthocyanin comparison, distinct from blueberry, metabolite association and mouse-injection experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Exact dose-series values not extracted from accessible abstract. Mouse metabolite dosing is not human mediation proof.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Purified anthocyanins improved FMD in healthy humans in the 2019 experimental program.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Circulating Anthocyanin Metabolites Mediate Vascular Benefits of Blueberries: Insights From Randomized Controlled Trials, Metabolomics, and Nutrigenomics. | 2019 | DOI 10.1093/gerona/glz047 | PMID 30772905 | https://pubmed.ncbi.nlm.nih.gov/30772905/ | https://doi.org/10.1093/gerona/glz047","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 56-56; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8efa7ad5-6736-5022-8c93-101f9d4955ce","evidence_kind":"source_excerpt","locator":"Lines 56-56","start_line":56,"end_line":56,"excerpt":"**Metabolite-mediated vascular activity has been tested.** A 2019 program compared purified anthocyanins, blueberries and control preparations in people, related circulating metabolites to FMD, and tested selected metabolite administration in mice. Purified anthocyanins improved FMD, while fiber/mineral/vitamin control drinks did not show the same effect. Human associations, mouse interventions and blood-cell gene-expression changes are different evidence layers. They support further metabolite research; they do not prove every correlated molecule is necessary or sufficient in humans. [Rodriguez-Mateos et al., 2019](https://pubmed.ncbi.nlm.nih.gov/30772905/).","model_system":"Human purified-anthocyanin comparison, distinct from blueberry, metabolite association and mouse-injection experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b08e7f7d-4d34-56d7-a185-2124f8d72b3c","stable_key":"import-35ec55a7-323c-5c28-979e-3bdafe9d5769","title":"Anthocyanins: detailed mechanisms of action (reviewed 4 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"7cff1a47fbd9c625412b84162b1c823004b4162b7c009f9a11d5807fb8e04ef9","revision_id":"1bd4fa42-bfeb-5148-8250-fc363d8c1de0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}