{"id":"5c3ac08e-da8e-5bcf-948a-582f06e241a5","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-mitochondrial-respiration","predicate":"increases_in_assay","statement":"Podzol-derived fulvic material increased respiration in isolated rat liver mitochondria.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"16e50ec4-cfce-5675-9838-e55d564ff7df","mechanism_event_label":"A soil-derived fraction changed respiration in isolated organelles.","subject":{"id":"7c9cabcf-f98c-550a-956d-188d907ec4e7","slug":"podzol-fulvic-1987","display_name":"Podzol-derived fulvic fraction, 1987 study","entity_type_key":"chemical_species"},"object":{"id":"f95104e8-89fc-5434-82f3-7b7feea4f9c1","slug":"rat-liver-fulvic-respiration","display_name":"Rat liver mitochondrial respiration after podzol fulvic exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"16e50ec4-cfce-5675-9838-e55d564ff7df","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-mitochondrial-respiration-event","event_type":"observed_relationship","label":"A soil-derived fraction changed respiration in isolated organelles.","description":"Podzol-derived fulvic material increased respiration in isolated rat liver mitochondria.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7c9cabcf-f98c-550a-956d-188d907ec4e7","slug":"podzol-fulvic-1987","display_name":"Podzol-derived fulvic fraction, 1987 study","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f95104e8-89fc-5434-82f3-7b7feea4f9c1","slug":"rat-liver-fulvic-respiration","display_name":"Rat liver mitochondrial respiration after podzol fulvic exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"In-vitro mitochondrial exposure, 40-360 mg/L; lower-molecular-weight fractions had larger effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No oral pharmacokinetics or human ATP benefit is demonstrated. The abstract groups humic substances when describing phosphorylation efficiency, so a fulvic-specific efficiency claim is not inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A soil-derived fraction changed respiration in isolated organelles.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effect of humic substances on mitochondrial respiration and oxidative phosphorylation. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2953069/ · DOI 10.1016/0048-9697(87)90521-3","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"35359311-97ad-5b47-b509-c87336daa0ce","evidence_kind":"source_excerpt","locator":"Lines 188-194","start_line":188,"end_line":194,"excerpt":"## fulvic-acid-mitochondrial-respiration\nA soil-derived fraction changed respiration in isolated organelles.\nPodzol-derived fulvic material increased respiration in isolated rat liver mitochondria.\nModel: In-vitro mitochondrial exposure, 40-360 mg/L; lower-molecular-weight fractions had larger effects.\nLimitations: No oral pharmacokinetics or human ATP benefit is demonstrated. The abstract groups humic substances when describing phosphorylation efficiency, so a fulvic-specific efficiency claim is not inferred.\nEvidence access: Primary abstract\nEffect of humic substances on mitochondrial respiration and oxidative phosphorylation. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2953069/ · DOI 10.1016/0048-9697(87)90521-3","model_system":"In-vitro mitochondrial exposure, 40-360 mg/L; lower-molecular-weight fractions had larger effects.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"52c2c3ee6bb23d943a40e1c39dd9de5cca7acd6efa72479cb824149e85cc7968","revision_id":"94dfc01b-c93e-55ff-8f48-bb2e9a1979f6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}