{"id":"5c389d00-6c14-5f4b-b1b7-1da5b2463168","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-srr-pc12-loss","predicate":"is_not_sole_requirement_for","statement":"Deleting endogenous SRR in rat PC12 cells lowered D-serine but left D-aspartate production unchanged.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3d8d7137-b764-5435-9b52-4ff703476d6b","mechanism_event_label":"The cells retained another way to maintain D-aspartate.","subject":{"id":"6bd7c01b-7d6b-5bad-a0a3-6fb892b9ba6e","slug":"rat-srr","display_name":"Rat serine racemase / Srr","entity_type_key":"protein"},"object":{"id":"6f410950-b5e0-57d2-8a42-5b754b138038","slug":"rat-pc12-d-aspartate-pool","display_name":"D-aspartate pool in rat PC12 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3d8d7137-b764-5435-9b52-4ff703476d6b","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-srr-pc12-loss-event","event_type":"observed_relationship","label":"The cells retained another way to maintain D-aspartate.","description":"Deleting endogenous SRR in rat PC12 cells lowered D-serine but left D-aspartate production unchanged.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6bd7c01b-7d6b-5bad-a0a3-6fb892b9ba6e","slug":"rat-srr","display_name":"Rat serine racemase / Srr","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6f410950-b5e0-57d2-8a42-5b754b138038","slug":"rat-pc12-d-aspartate-pool","display_name":"D-aspartate pool in rat PC12 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7f3bf9f5-b1b1-5381-baa6-72800ceb5436","slug":"d-serine","display_name":"D-Serine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat PC12 SRR knockout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The alternative synthesis route was not identified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The cells retained another way to maintain D-aspartate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Serine racemase is involved in d-aspartate biosynthesis. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27387750/ · DOI 10.1093/jb/mvw043","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c43a6983-3d1c-5586-8903-77df5efbc5d7","evidence_kind":"source_excerpt","locator":"Lines 32-38","start_line":32,"end_line":38,"excerpt":"## d-aspartate-srr-pc12-loss\nThe cells retained another way to maintain D-aspartate.\nDeleting endogenous SRR in rat PC12 cells lowered D-serine but left D-aspartate production unchanged.\nModel: Rat PC12 SRR knockout.\nLimitations: The alternative synthesis route was not identified.\nEvidence access: Primary abstract\nSerine racemase is involved in d-aspartate biosynthesis. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27387750/ · DOI 10.1093/jb/mvw043","model_system":"Rat PC12 SRR knockout.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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