{"id":"5c058cf1-0285-5e9b-a4e4-4373ed53b2d2","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-npc1l1-null","predicate":"inhibition_did_not_reduce","statement":"NPC1L1 antibody and ezetimibe did not significantly reduce all-E or 5Z lycopene uptake in Caco-2 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"122ba16b-24ab-5e7d-8a2b-2f4b38627956","mechanism_event_label":"A transporter used by other lipids was not necessary in this particular assay.","subject":{"id":"9fbf0133-53c3-5ab1-8a95-4804d6115300","slug":"npc1l1","display_name":"Human Niemann-Pick C1-like protein 1 / NPC1L1","entity_type_key":"protein"},"object":{"id":"1965b03d-da13-5707-8612-d6bce4b5f53f","slug":"intestinal-lycopene-uptake","display_name":"Intestinal epithelial lycopene uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"122ba16b-24ab-5e7d-8a2b-2f4b38627956","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-npc1l1-null-event","event_type":"biochemical_relationship","label":"A transporter used by other lipids was not necessary in this particular assay.","description":"NPC1L1 antibody and ezetimibe did not significantly reduce all-E or 5Z lycopene uptake in Caco-2 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4a88840d-be05-5442-9938-a1da9df4d385","slug":"all-trans-lycopene","display_name":"All-trans-lycopene / all-E-lycopene","entity_type_key":"small_molecule"},"role":"tested_isomer","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"19c32171-1fe6-55f3-9d31-6c442e4a423d","slug":"5-cis-lycopene","display_name":"5-cis-Lycopene / 5Z-lycopene","entity_type_key":"small_molecule"},"role":"tested_isomer","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9fbf0133-53c3-5ab1-8a95-4804d6115300","slug":"npc1l1","display_name":"Human Niemann-Pick C1-like protein 1 / NPC1L1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1965b03d-da13-5707-8612-d6bce4b5f53f","slug":"intestinal-lycopene-uptake","display_name":"Intestinal epithelial lycopene uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/18641187.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7c71fd4fd3be9d14176bceb3c2104143269a788587feec232350a6f1c5913e3d\", \"start_char\": 0, \"end_char\": 1557, \"text_sha256\": \"7c71fd4fd3be9d14176bceb3c2104143269a788587feec232350a6f1c5913e3d\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transporter inhibition in Caco-2 cells; intestinal SR-BI transgenic mice","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"All-E and 5Z lycopene; mice received 0.25 g/kg diet for one month","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Partial uptake inhibition is not proof of a sole transporter. Ezetimibe result is a cell assay, not a clinical drug-interaction trial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human Caco-2 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A transporter used by other lipids was not necessary in this particular assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p18641187] Lycopene absorption in human intestinal cells and in mice involves scavenger receptor class B type I but not Niemann-Pick C1-like 1. (2008). https://pubmed.ncbi.nlm.nih.gov/18641187/ DOI: 10.1093/jn/138.8.1432","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal epithelium and plasma","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7506d9b9-7ac3-589b-8256-9ca842789253","evidence_kind":"source_excerpt","locator":"Lines 117-128","start_line":117,"end_line":128,"excerpt":"### lycopene-npc1l1-null\nNPC1L1 antibody and ezetimibe did not significantly reduce all-E or 5Z lycopene uptake in Caco-2 cells.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A transporter used by other lipids was not necessary in this particular assay.\norganism: Human Caco-2 cells\ntissue_or_cell_type: Intestinal epithelium and plasma\nexperimental_model: Transporter inhibition in Caco-2 cells; intestinal SR-BI transgenic mice\nlimitations: Partial uptake inhibition is not proof of a sole transporter. Ezetimibe result is a cell assay, not a clinical drug-interaction trial.\nexposure: All-E and 5Z lycopene; mice received 0.25 g/kg diet for one month\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/18641187.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7c71fd4fd3be9d14176bceb3c2104143269a788587feec232350a6f1c5913e3d\", \"start_char\": 0, \"end_char\": 1557, \"text_sha256\": \"7c71fd4fd3be9d14176bceb3c2104143269a788587feec232350a6f1c5913e3d\"}\n[lycopene-p18641187] Lycopene absorption in human intestinal cells and in mice involves scavenger receptor class B type I but not Niemann-Pick C1-like 1. (2008). https://pubmed.ncbi.nlm.nih.gov/18641187/ DOI: 10.1093/jn/138.8.1432","model_system":"Transporter inhibition in Caco-2 cells; intestinal SR-BI transgenic mice","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lycopene-p18641187] Lycopene absorption in human intestinal cells and in mice involves scavenger receptor class B type I but not Niemann-Pick C1-like 1. (2008). https://pubmed.ncbi.nlm.nih.gov/18641187/ DOI: 10.1093/jn/138.8.1432","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d1e09874-8bde-548f-9664-26ad5eedb3c7","stable_key":"import-aad5a443-96e9-52eb-963a-812e90f9f896","title":"Lycopene: absorption, metabolism, nutrient connections and human outcomes (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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