{"id":"5bb7158b-258f-5958-8ae0-1235a7212310","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-vrac-taurine","predicate":"knockdown_reduces","statement":"RNAi knockdown of Lrrc8d strongly reduced swelling-evoked taurine release from primary rat astrocytes; Lrrc8a knockdown also suppressed release.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5b903956-7d85-58b0-8abd-b405d761c16d","mechanism_event_label":"Swollen brain-support cells release taurine through a channel with specific subunits.","subject":{"id":"a33b79dc-5055-5a1e-8557-cd53c4db503c","slug":"rat-lrrc8d","display_name":"Rat LRRC8D / Lrrc8d","entity_type_key":"protein"},"object":{"id":"cf8be54d-3108-5b36-8cee-6e586a529348","slug":"rat-taurine-efflux","display_name":"Rat astrocyte swelling-evoked taurine efflux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5b903956-7d85-58b0-8abd-b405d761c16d","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-vrac-taurine-event","event_type":"observed_relationship","label":"Swollen brain-support cells release taurine through a channel with specific subunits.","description":"RNAi knockdown of Lrrc8d strongly reduced swelling-evoked taurine release from primary rat astrocytes; Lrrc8a knockdown also suppressed release.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a33b79dc-5055-5a1e-8557-cd53c4db503c","slug":"rat-lrrc8d","display_name":"Rat LRRC8D / Lrrc8d","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cf8be54d-3108-5b36-8cee-6e586a529348","slug":"rat-taurine-efflux","display_name":"Rat astrocyte swelling-evoked taurine efflux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bcfb8b83-7eb6-54a0-a289-f5546afe6769","slug":"rat-lrrc8a","display_name":"Rat LRRC8A / Lrrc8a","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary rat astrocytes; 30% hypoosmotic challenge and radiotracers.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"RNAi reduction, not whole-animal gene deletion; this is efflux rather than SLC6A6 uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Swollen brain-support cells release taurine through a channel with specific subunits.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Molecular composition and heterogeneity of the LRRC8-containing swelling-activated osmolyte channels in primary rat astrocytes. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28833202/ · DOI 10.1113/JP275053","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6625852b-f161-5acb-88a5-ba2d08366b12","evidence_kind":"source_excerpt","locator":"Lines 401-407","start_line":401,"end_line":407,"excerpt":"## taurine-vrac-taurine\nSwollen brain-support cells release taurine through a channel with specific subunits.\nRNAi knockdown of Lrrc8d strongly reduced swelling-evoked taurine release from primary rat astrocytes; Lrrc8a knockdown also suppressed release.\nModel: Primary rat astrocytes; 30% hypoosmotic challenge and radiotracers.\nLimitations: RNAi reduction, not whole-animal gene deletion; this is efflux rather than SLC6A6 uptake.\nEvidence access: Primary abstract\nMolecular composition and heterogeneity of the LRRC8-containing swelling-activated osmolyte channels in primary rat astrocytes. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28833202/ · DOI 10.1113/JP275053","model_system":"Primary rat astrocytes; 30% hypoosmotic challenge and radiotracers.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}