{"id":"5b8e4dfd-103a-5ccd-b6cc-24418b4cc5e5","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars1-translation-scaffold","predicate":"assembles_with","statement":"Threonyl-tRNA synthetase interacted with 4EHP and recruited initiation components into a translation-initiation machinery that selected target mRNAs.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fbe4b314-acc4-5f06-8f93-dbfe889f0361","mechanism_event_label":"A tRNA-loading enzyme can also help organize translation initiation.","subject":{"id":"42b7656b-93a3-5348-a7cf-775072512653","slug":"tars1","display_name":"Human cytosolic threonyl-tRNA synthetase / TARS1","entity_type_key":"protein"},"object":{"id":"fb702a02-874b-5b89-963c-c9527323e626","slug":"eif4e2","display_name":"Human eIF4E homologous protein / 4EHP / EIF4E2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"fbe4b314-acc4-5f06-8f93-dbfe889f0361","stable_key":"97957230-601f-5dec-8524-0812be8fadbf:l-threonine-tars1-translation-scaffold-event","event_type":"observed_relationship","label":"A tRNA-loading enzyme can also help organize translation initiation.","description":"Threonyl-tRNA synthetase interacted with 4EHP and recruited initiation components into a translation-initiation machinery that selected target mRNAs.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"42b7656b-93a3-5348-a7cf-775072512653","slug":"tars1","display_name":"Human cytosolic threonyl-tRNA synthetase / TARS1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fb702a02-874b-5b89-963c-c9527323e626","slug":"eif4e2","display_name":"Human eIF4E homologous protein / 4EHP / EIF4E2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fcef4dc2-a7b6-5812-bc33-c8af3d83f4d0","slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Biochemical interaction and RNA immunoprecipitation/sequencing analyses in vertebrate models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This scaffold function is not evidence that adding free threonine increases translation of the same targets.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Threonine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-threonine","display_name":"L-Threonine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A tRNA-loading enzyme can also help organize translation initiation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A threonyl-tRNA synthetase-mediated translation initiation machinery. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30902983/ · DOI 10.1038/s41467-019-09086-0","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b1914283-2976-597f-bb87-a2e6ed815a80","evidence_kind":"source_excerpt","locator":"Lines 178-184","start_line":178,"end_line":184,"excerpt":"## l-threonine-tars1-translation-scaffold\nA tRNA-loading enzyme can also help organize translation initiation.\nThreonyl-tRNA synthetase interacted with 4EHP and recruited initiation components into a translation-initiation machinery that selected target mRNAs.\nModel: Biochemical interaction and RNA immunoprecipitation/sequencing analyses in vertebrate models.\nLimitations: This scaffold function is not evidence that adding free threonine increases translation of the same targets.\nEvidence access: Primary abstract\nA threonyl-tRNA synthetase-mediated translation initiation machinery. · 2019 · https://pubmed.ncbi.nlm.nih.gov/30902983/ · DOI 10.1038/s41467-019-09086-0","model_system":"Biochemical interaction and RNA immunoprecipitation/sequencing analyses in vertebrate models.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"35396730-235a-537e-8a05-c6ef2960c509","stable_key":"import-97957230-601f-5dec-8524-0812be8fadbf","title":"L-Threonine: translation, intestinal barrier, metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3365aed939d9f567bd098449c7d67c7c00fb6c9ba163dcf34dc7cf6081f324fb","revision_id":"10a7b648-8dd7-5d4e-b618-c560a6e2d3c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}