{"id":"5b8707d7-5387-50c5-b2bd-2781626cb8b1","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-breg-silencing-txn1","predicate":"silencing_reduces","statement":"CRISPR–Cas9 silencing of TXN1 reduced IL-10-positive B-cell frequencies after CpGC stimulation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5a78caa4-4830-58f9-8bff-30478b6f6bd6","mechanism_event_label":"Removing one component weakens the same immune program.","subject":{"id":"acb6f128-e816-5d81-babc-db2ecbe93328","slug":"txn1","display_name":"TXN1","entity_type_key":"protein"},"object":{"id":"d7d00d06-34cf-5c76-9f8b-124726657a6a","slug":"human-il10-breg-differentiation","display_name":"Human IL-10-positive regulatory B-cell differentiation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5a78caa4-4830-58f9-8bff-30478b6f6bd6","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-breg-silencing-txn1-event","event_type":"observed_relationship","label":"Removing one component weakens the same immune program.","description":"CRISPR–Cas9 silencing of TXN1 reduced IL-10-positive B-cell frequencies after CpGC stimulation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"acb6f128-e816-5d81-babc-db2ecbe93328","slug":"txn1","display_name":"TXN1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d7d00d06-34cf-5c76-9f8b-124726657a6a","slug":"human-il10-breg-differentiation","display_name":"Human IL-10-positive regulatory B-cell differentiation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human B cells; 72-hour stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Supports machinery dependence, not selective drug targeting or evidence that a supplement causes autoimmunity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing one component weakens the same immune program.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Thioredoxin is a metabolic rheostat controlling regulatory B cells. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38553615/ · DOI 10.1038/s41590-024-01798-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"890912e8-9d25-50e5-878a-60a0ec543049","evidence_kind":"source_excerpt","locator":"Lines 308-314","start_line":308,"end_line":314,"excerpt":"## myricetin-breg-silencing-txn1\nRemoving one component weakens the same immune program.\nCRISPR–Cas9 silencing of TXN1 reduced IL-10-positive B-cell frequencies after CpGC stimulation.\nModel: Primary human B cells; 72-hour stimulation.\nLimitations: Supports machinery dependence, not selective drug targeting or evidence that a supplement causes autoimmunity.\nEvidence access: Primary full text\nThioredoxin is a metabolic rheostat controlling regulatory B cells. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38553615/ · DOI 10.1038/s41590-024-01798-w","model_system":"Primary human B cells; 72-hour stimulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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