{"id":"5b4671cc-36be-5043-996d-45c465f55cf3","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:aldh1l2-coa-arm","predicate":"modifies","statement":"Human phosphopantetheinyl transferase transfers a CoA-derived prosthetic group to ALDH1L2 Ser375.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"eca5e709-4bbd-594a-a53b-540873714cde","mechanism_event_label":"A CoA-derived arm prepares the folate enzyme for catalysis.","subject":{"id":"df1ba694-d4ab-5a09-adac-272f9f9df60d","slug":"aasdhppt","display_name":"Human 4-phosphopantetheinyl transferase / AASDHPPT","entity_type_key":"protein"},"object":{"id":"8079aee1-6515-5733-80fa-e243016e3b4a","slug":"aldh1l2","display_name":"Human ALDH1L2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"eca5e709-4bbd-594a-a53b-540873714cde","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:aldh1l2-coa-arm-event","event_type":"biochemical_relationship","label":"A CoA-derived arm prepares the folate enzyme for catalysis.","description":"Human phosphopantetheinyl transferase transfers a CoA-derived prosthetic group to ALDH1L2 Ser375.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"prosthetic-group donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"751e0dae-384b-5ce5-8365-abd7939b1474","slug":"aldh1l2-ser375-phosphopantetheinyl","display_name":"Human ALDH1L2 phosphopantetheinylated at Ser375","entity_type_key":"protein_state"},"role":"modified product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7bc62072-9517-5563-a3b8-8c5ab64a464b","slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"},"role":"upstream CoA nutrient dependency","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"df1ba694-d4ab-5a09-adac-272f9f9df60d","slug":"aasdhppt","display_name":"Human 4-phosphopantetheinyl transferase / AASDHPPT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8079aee1-6515-5733-80fa-e243016e3b4a","slug":"aldh1l2","display_name":"Human ALDH1L2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B5-derived CoA supplies phosphopantetheine; direct CoA handoff tested, dietary B5 link upstream.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reconstitution and site-directed mutagenesis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish dietary B5 deficiency or repletion effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A CoA-derived arm prepares the folate enzyme for catalysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[strickland-2011] Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase (2011). https://pubmed.ncbi.nlm.nih.gov/21238436/ DOI: 10.1016/j.cbi.2011.01.008","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ee4dc57c-3f88-5360-a7a5-1141b8387de7","evidence_kind":"source_excerpt","locator":"Lines 1046-1057","start_line":1046,"end_line":1057,"excerpt":"### aldh1l2-coa-arm\nHuman phosphopantetheinyl transferase transfers a CoA-derived prosthetic group to ALDH1L2 Ser375.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A CoA-derived arm prepares the folate enzyme for catalysis.\norganism: Homo sapiens\ntissue_or_cell_type: Cell-free\nexperimental_model: Reconstitution and site-directed mutagenesis\nlimitations: Does not establish dietary B5 deficiency or repletion effects.\nexposure: Assay conditions described in the linked primary study.\ncross_nutrient: B5-derived CoA supplies phosphopantetheine; direct CoA handoff tested, dietary B5 link upstream.\n[strickland-2011] Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase (2011). https://pubmed.ncbi.nlm.nih.gov/21238436/ DOI: 10.1016/j.cbi.2011.01.008","model_system":"Reconstitution and site-directed mutagenesis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [strickland-2011] Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase (2011). https://pubmed.ncbi.nlm.nih.gov/21238436/ DOI: 10.1016/j.cbi.2011.01.008","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}