{"id":"5b432964-cb52-548c-8339-e8918086c81d","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-stc-gpr142","predicate":"signals_through","statement":"In STC-1 enteroendocrine cells, phenylalanine stimulated a GPR142/Gq-linked calcium response contributing to GLP-1 release.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"bd85cf06-942a-51b1-b328-661f2d6b5f1e","mechanism_event_label":"A gut-cell model used a receptor-to-calcium pathway to release a hormone.","subject":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"object":{"id":"a1317efc-1f28-5b59-be4d-1f9b051120af","slug":"mouse-gpr142","display_name":"Mouse Gpr142","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bd85cf06-942a-51b1-b328-661f2d6b5f1e","stable_key":"56f1d914-e7da-595a-af69-c217b2b47407:l-phenylalanine-stc-gpr142-event","event_type":"observed_relationship","label":"A gut-cell model used a receptor-to-calcium pathway to release a hormone.","description":"In STC-1 enteroendocrine cells, phenylalanine stimulated a GPR142/Gq-linked calcium response contributing to GLP-1 release.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a1317efc-1f28-5b59-be4d-1f9b051120af","slug":"mouse-gpr142","display_name":"Mouse Gpr142","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4853afc6-9eaa-547f-91d7-c4dd90082351","slug":"glp-1","display_name":"Glucagon-like peptide 1 / GLP-1","entity_type_key":"peptide"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse STC-1 cells, live-cell signaling and pharmacologic experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Exposure details beyond the accessed abstract are unresolved; this is not direct human gut evidence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Phenylalanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A gut-cell model used a receptor-to-calcium pathway to release a hormone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Identification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34953208/ · DOI 10.1016/j.bbrc.2021.12.043","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"62437f43-2810-5f1b-b6b9-2a99685aca3f","evidence_kind":"source_excerpt","locator":"Lines 310-316","start_line":310,"end_line":316,"excerpt":"## l-phenylalanine-stc-gpr142\nA gut-cell model used a receptor-to-calcium pathway to release a hormone.\nIn STC-1 enteroendocrine cells, phenylalanine stimulated a GPR142/Gq-linked calcium response contributing to GLP-1 release.\nModel: Mouse STC-1 cells, live-cell signaling and pharmacologic experiments.\nLimitations: Exposure details beyond the accessed abstract are unresolved; this is not direct human gut evidence.\nEvidence access: Primary abstract\nIdentification of a regulatory pathway of L-phenylalanine-induced GLP-1 secretion in the enteroendocrine L cells. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34953208/ · DOI 10.1016/j.bbrc.2021.12.043","model_system":"Mouse STC-1 cells, live-cell signaling and pharmacologic experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54de7c56-1e60-5d7b-8ef9-852c58ae92ba","stable_key":"import-56f1d914-e7da-595a-af69-c217b2b47407","title":"L-Phenylalanine: transport, protein synthesis, cofactor recycling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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