{"id":"5ac48d25-0944-5170-aa4f-4c48a35de92a","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-p53-gls2","predicate":"induces","statement":"Activating p53 increased GLS2 expression under stressed and unstressed conditions in the reported human-cell experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b0e45afe-cba8-5ce0-b934-8f4e3c79c2c2","mechanism_event_label":"Gene regulation changes the machinery that supplies glutamate.","subject":{"id":"74fc9826-1f74-545e-86db-fe687794f70d","slug":"tp53","display_name":"Human tumor protein p53 / TP53","entity_type_key":"protein"},"object":{"id":"6981fb90-5bc3-5c2a-9f22-f1974e287f38","slug":"gls2","display_name":"Human glutaminase 2 / GLS2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"b0e45afe-cba8-5ce0-b934-8f4e3c79c2c2","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-p53-gls2-event","event_type":"observed_relationship","label":"Gene regulation changes the machinery that supplies glutamate.","description":"Activating p53 increased GLS2 expression under stressed and unstressed conditions in the reported human-cell experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"74fc9826-1f74-545e-86db-fe687794f70d","slug":"tp53","display_name":"Human tumor protein p53 / TP53","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6981fb90-5bc3-5c2a-9f22-f1974e287f38","slug":"gls2","display_name":"Human glutaminase 2 / GLS2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell p53 and GLS2 expression/perturbation assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A tumor-suppressor pathway is not evidence that glutamate supplementation prevents cancer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Glutamate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Gene regulation changes the machinery that supplies glutamate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20378837/ · DOI 10.1073/pnas.1001006107","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8e33d5ab-386d-50fa-b3b4-45ce71c8ed1d","evidence_kind":"source_excerpt","locator":"Lines 210-216","start_line":210,"end_line":216,"excerpt":"## glutamate-p53-gls2\nGene regulation changes the machinery that supplies glutamate.\nActivating p53 increased GLS2 expression under stressed and unstressed conditions in the reported human-cell experiments.\nModel: Human-cell p53 and GLS2 expression/perturbation assays.\nLimitations: A tumor-suppressor pathway is not evidence that glutamate supplementation prevents cancer.\nEvidence access: Primary abstract\nGlutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20378837/ · DOI 10.1073/pnas.1001006107","model_system":"Human-cell p53 and GLS2 expression/perturbation assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c6a55239-e808-5ed7-9fa3-755a18ecb29f","stable_key":"import-b7014def-74ec-599e-8347-167e24fec1f0","title":"L-Glutamate / L-glutamic acid: carbon and nitrogen allocation, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"3da83bd75fddeb96d17af72d7e2ae04344779957650f266521f6c8ebd59fb54f","revision_id":"55196c72-94e4-540a-a869-81d35647b04a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}