{"id":"5a808665-2dab-5cc1-a512-86841a824588","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-ipmk-ip4","predicate":"phosphorylates","statement":"Human IPMK has IP3 3-kinase activity, producing inositol 1,3,4,5-tetrakisphosphate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"501d7b08-7c52-5b78-9cba-a6712b3fba02","mechanism_event_label":"Soluble IP3 can be redirected into a more highly phosphorylated molecule.","subject":{"id":"36d24d17-0994-51e9-82ce-2c2a3b2559e8","slug":"ipmk","display_name":"IPMK (human inositol phosphate multikinase)","entity_type_key":"protein"},"object":{"id":"7005bbc2-d4b5-5903-b776-94dd2288d35f","slug":"ip3","display_name":"IP3","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"501d7b08-7c52-5b78-9cba-a6712b3fba02","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-ipmk-ip4-event","event_type":"biochemical_relationship","label":"Soluble IP3 can be redirected into a more highly phosphorylated molecule.","description":"Human IPMK has IP3 3-kinase activity, producing inositol 1,3,4,5-tetrakisphosphate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0c6f4f5c-eede-5c0d-a5d7-b45cc940da37","slug":"inositol-1345-tetrakisphosphate","display_name":"Inositol 1,3,4,5-tetrakisphosphate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"36d24d17-0994-51e9-82ce-2c2a3b2559e8","slug":"ipmk","display_name":"IPMK (human inositol phosphate multikinase)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7005bbc2-d4b5-5903-b776-94dd2288d35f","slug":"ip3","display_name":"IP3","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/28882892.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"39494635d8bd1a8a8df3ac71f8aa6c5f6440bd3c6fa7cb4756e52d572a8f16db\", \"start_char\": 0, \"end_char\": 1781, \"text_sha256\": \"39494635d8bd1a8a8df3ac71f8aa6c5f6440bd3c6fa7cb4756e52d572a8f16db\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Crystal structures and targeted human IPMK mutants","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"IP3 and PI(4,5)P2 substrate binding and phosphorylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human substrate specificity differs from yeast and plant orthologs; do not merge their regioselectivity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Soluble IP3 can be redirected into a more highly phosphorylated molecule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p28882892] Structural features of human inositol phosphate multikinase rationalize its inositol phosphate kinase and phosphoinositide 3-kinase activities. (2017). https://pubmed.ncbi.nlm.nih.gov/28882892/ DOI: 10.1074/jbc.m117.801845","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified IPMK","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1663c1f4-ef6c-5ec3-a9d1-c6a971d0efc0","evidence_kind":"source_excerpt","locator":"Lines 678-689","start_line":678,"end_line":689,"excerpt":"### ino-ipmk-ip4\nHuman IPMK has IP3 3-kinase activity, producing inositol 1,3,4,5-tetrakisphosphate.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Soluble IP3 can be redirected into a more highly phosphorylated molecule.\norganism: Human protein\ntissue_or_cell_type: Purified IPMK\nexperimental_model: Crystal structures and targeted human IPMK mutants\nlimitations: Human substrate specificity differs from yeast and plant orthologs; do not merge their regioselectivity.\nexposure: IP3 and PI(4,5)P2 substrate binding and phosphorylation\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/28882892.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"39494635d8bd1a8a8df3ac71f8aa6c5f6440bd3c6fa7cb4756e52d572a8f16db\", \"start_char\": 0, \"end_char\": 1781, \"text_sha256\": \"39494635d8bd1a8a8df3ac71f8aa6c5f6440bd3c6fa7cb4756e52d572a8f16db\"}\n[ino-p28882892] Structural features of human inositol phosphate multikinase rationalize its inositol phosphate kinase and phosphoinositide 3-kinase activities. (2017). https://pubmed.ncbi.nlm.nih.gov/28882892/ DOI: 10.1074/jbc.m117.801845","model_system":"Crystal structures and targeted human IPMK mutants","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p28882892] Structural features of human inositol phosphate multikinase rationalize its inositol phosphate kinase and phosphoinositide 3-kinase activities. (2017). https://pubmed.ncbi.nlm.nih.gov/28882892/ DOI: 10.1074/jbc.m117.801845","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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