{"id":"5a58bc36-57b4-550f-92b4-bd385fb3d246","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-agmat-gba","predicate":"hydrolyzes","statement":"Both purified human AGMAT variants hydrolyzed guanidinobutyrate, supporting a guanidino-acid hydrolase function.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6610b757-4fee-538d-84ca-73c91e41df0d","mechanism_event_label":"The same protein was active when supplied a different substrate.","subject":{"id":"bba88813-506c-5bbf-b85e-fc33beefc748","slug":"agmat","display_name":"Human AGMAT, guanidino acid hydrolase candidate / historically annotated agmatinase","entity_type_key":"protein"},"object":{"id":"508e25ee-c4f9-53ac-9b9b-3b122a90c6d9","slug":"4-guanidinobutyrate","display_name":"4-Guanidinobutyrate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6610b757-4fee-538d-84ca-73c91e41df0d","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-agmat-gba-event","event_type":"observed_relationship","label":"The same protein was active when supplied a different substrate.","description":"Both purified human AGMAT variants hydrolyzed guanidinobutyrate, supporting a guanidino-acid hydrolase function.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bba88813-506c-5bbf-b85e-fc33beefc748","slug":"agmat","display_name":"Human AGMAT, guanidino acid hydrolase candidate / historically annotated agmatinase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"508e25ee-c4f9-53ac-9b9b-3b122a90c6d9","slug":"4-guanidinobutyrate","display_name":"4-Guanidinobutyrate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d5b98fa8-0612-5a5a-aa3c-6cfd8ff88867","slug":"gaba","display_name":"Gamma-aminobutyric acid","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"37cf0152-db25-5773-a726-d0c0facb2e24","slug":"urea","display_name":"Urea","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Figures 2 and 3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified recombinant human R105 and G105 variants.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Measured Km values for the tested guanidino acids exceeded 50 mM; physiological substrate flux remains unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same protein was active when supplied a different substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Guanidino acid hydrolysis by the human enzyme annotated as agmatinase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36543883/ · DOI 10.1038/s41598-022-26655-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8fd96730-2935-5a4f-ae90-606a1aa86c48","evidence_kind":"source_excerpt","locator":"Lines 124-130","start_line":124,"end_line":130,"excerpt":"## agmatine-sulfate-agmat-gba\nThe same protein was active when supplied a different substrate.\nBoth purified human AGMAT variants hydrolyzed guanidinobutyrate, supporting a guanidino-acid hydrolase function.\nModel: Purified recombinant human R105 and G105 variants.\nLimitations: Measured Km values for the tested guanidino acids exceeded 50 mM; physiological substrate flux remains unresolved.\nEvidence access: Primary full text; Figures 2 and 3\nGuanidino acid hydrolysis by the human enzyme annotated as agmatinase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36543883/ · DOI 10.1038/s41598-022-26655-4","model_system":"Purified recombinant human R105 and G105 variants.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b55d70e22d07b80964f825770d92fdd1745221420ed8af8d94ca5182b9994509","revision_id":"fc7be029-20e0-5943-b74e-3b06ba694571","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}