{"id":"59f69c13-d84d-5f67-b0da-c8babb0fb7f2","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-human-marco-binding","predicate":"supports_silica_binding","statement":"Human MARCO expression in CHO cells supported silica binding and apoptosis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ede84a8d-2de9-5f5b-986b-ac0dd37ee9ad","mechanism_event_label":"A human receptor was tested in a nonhuman host cell.","subject":{"id":"845959f6-edc8-5f63-bb6d-7090fee99c20","slug":"human-marco","display_name":"Human MARCO","entity_type_key":"protein"},"object":{"id":"19cec617-93c6-51b4-8db2-aa5c5d61dd02","slug":"human-marco-transfected-cho-response","display_name":"Response of human-MARCO-transfected hamster cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ede84a8d-2de9-5f5b-986b-ac0dd37ee9ad","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-human-marco-binding-event","event_type":"observed_relationship","label":"A human receptor was tested in a nonhuman host cell.","description":"Human MARCO expression in CHO cells supported silica binding and apoptosis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"845959f6-edc8-5f63-bb6d-7090fee99c20","slug":"human-marco","display_name":"Human MARCO","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"19cec617-93c6-51b4-8db2-aa5c5d61dd02","slug":"human-marco-transfected-cho-response","display_name":"Response of human-MARCO-transfected hamster cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b2d4231b-3893-5d4e-8b52-d0effdaa26c9","slug":"crystalline-silica","display_name":"Crystalline silica / quartz particles","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human construct in Chinese hamster ovary cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not direct observation of human lung exposure or a universal uptake route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A human receptor was tested in a nonhuman host cell.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"MARCO mediates silica uptake and toxicity in alveolar macrophages from C57BL/6 mice. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16984918/ · DOI 10.1074/jbc.M605229200","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3aa94eda-c455-591a-8b9f-f3aee531b2cd","evidence_kind":"source_excerpt","locator":"Lines 416-422","start_line":416,"end_line":422,"excerpt":"## silica-human-marco-binding\nA human receptor was tested in a nonhuman host cell.\nHuman MARCO expression in CHO cells supported silica binding and apoptosis.\nModel: Human construct in Chinese hamster ovary cells.\nLimitations: Not direct observation of human lung exposure or a universal uptake route.\nEvidence access: Primary abstract\nMARCO mediates silica uptake and toxicity in alveolar macrophages from C57BL/6 mice. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16984918/ · DOI 10.1074/jbc.M605229200","model_system":"Human construct in Chinese hamster ovary cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"e05d81962a1ca6cbfc68fce4fd3dbcdea67c8e61332c19f4fec5d0c3ab8bf1b9","revision_id":"6433ed49-a331-571b-a611-21e56c3ce4bc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}