{"id":"59eb0c0e-058e-517a-84e3-1372d22c2d02","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-irs1","predicate":"reduced","statement":"Lycopene reduced IGF-I-stimulated IRS1 tyrosine phosphorylation in MCF7 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"cf0c92ae-79a7-5c33-8cda-9fe25fdac532","mechanism_event_label":"The growth signal was weaker at an intracellular relay.","subject":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"object":{"id":"03361ccb-89c8-51b6-a3ce-193af30a30c7","slug":"breast-cancer-irs1-phosphorylation","display_name":"IRS1 tyrosine phosphorylation in human MCF7 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cf0c92ae-79a7-5c33-8cda-9fe25fdac532","stable_key":"aad5a443-96e9-52eb-963a-812e90f9f896:lycopene-irs1-event","event_type":"biochemical_relationship","label":"The growth signal was weaker at an intracellular relay.","description":"Lycopene reduced IGF-I-stimulated IRS1 tyrosine phosphorylation in MCF7 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1d558783-9f80-531e-b09f-4c69e08ac426","slug":"irs1","display_name":"Human insulin receptor substrate 1 / IRS1","entity_type_key":"protein"},"role":"intracellular_relay","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d240f5b5-d225-5f4a-ab28-8070ccb52870","slug":"igf1r","display_name":"Human insulin-like growth factor 1 receptor / IGF1R","entity_type_key":"protein"},"role":"growth_factor_receptor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0b16e027-7a68-55ae-8e33-eae8b5cd1e9e","slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"03361ccb-89c8-51b6-a3ce-193af30a30c7","slug":"breast-cancer-irs1-phosphorylation","display_name":"IRS1 tyrosine phosphorylation in human MCF7 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lycopene-research/10798222.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f9229468bbed86c3c4fdbb24f80d139f5cac41e1692195090d19a195bcd144e8\", \"start_char\": 0, \"end_char\": 1731, \"text_sha256\": \"f9229468bbed86c3c4fdbb24f80d139f5cac41e1692195090d19a195bcd144e8\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Growth-factor stimulation and signaling assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"IGF-I stimulation with lycopene exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-line mechanism; not proof of lowered human serum IGF-I or cancer treatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lycopene research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lycopene","display_name":"Lycopene","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human MCF7 breast cancer cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The growth signal was weaker at an intracellular relay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lycopene-p10798222] Lycopene interferes with cell cycle progression and insulin-like growth factor I signaling in mammary cancer cells. (2000). https://pubmed.ncbi.nlm.nih.gov/10798222/ DOI: 10.1207/s15327914nc3601_14","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"IRS1 phosphorylation, AP-1 and cell cycle","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c4db8d8d-6806-5948-85a2-733513874221","evidence_kind":"source_excerpt","locator":"Lines 663-674","start_line":663,"end_line":674,"excerpt":"### lycopene-irs1\nLycopene reduced IGF-I-stimulated IRS1 tyrosine phosphorylation in MCF7 cells.\nCondition category: normal\nnutrient_topic: Lycopene research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The growth signal was weaker at an intracellular relay.\norganism: Human MCF7 breast cancer cells\ntissue_or_cell_type: IRS1 phosphorylation, AP-1 and cell cycle\nexperimental_model: Growth-factor stimulation and signaling assays\nlimitations: Cell-line mechanism; not proof of lowered human serum IGF-I or cancer treatment.\nexposure: IGF-I stimulation with lycopene exposure\nevidence_span: {\"source_cache\": \"artifacts/lycopene-research/10798222.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"f9229468bbed86c3c4fdbb24f80d139f5cac41e1692195090d19a195bcd144e8\", \"start_char\": 0, \"end_char\": 1731, \"text_sha256\": \"f9229468bbed86c3c4fdbb24f80d139f5cac41e1692195090d19a195bcd144e8\"}\n[lycopene-p10798222] Lycopene interferes with cell cycle progression and insulin-like growth factor I signaling in mammary cancer cells. 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