{"id":"59b8d5f1-d2e7-5267-bb69-2c2fa7972692","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-adipose-pde3b","predicate":"reduces_transcript","statement":"Adipose PDE3B transcript levels decreased after fasting, with and without GH blockade.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d3bcf04c-3112-5852-85cd-83b61d547ff2","mechanism_event_label":"A molecular brake on fat release changed.","subject":{"id":"2d5df4b9-44d7-56a5-8ecf-7c08c0ae9f4f","slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"},"object":{"id":"79a0a128-8b97-570f-8b8b-dd28f2783375","slug":"pde3b","display_name":"Human phosphodiesterase 3B / PDE3B","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d3bcf04c-3112-5852-85cd-83b61d547ff2","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-adipose-pde3b-event","event_type":"observed_relationship","label":"A molecular brake on fat release changed.","description":"Adipose PDE3B transcript levels decreased after fasting, with and without GH blockade.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2d5df4b9-44d7-56a5-8ecf-7c08c0ae9f4f","slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"79a0a128-8b97-570f-8b8b-dd28f2783375","slug":"pde3b","display_name":"Human phosphodiesterase 3B / PDE3B","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"de39736b-f86e-5e84-9616-bf28f52850ab","slug":"whole-body-lipolytic-flux","display_name":"Whole-body lipolytic flux","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full-text Results: Regulation of lipolysis, DOI 10.1210/jc.2016-3835","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Nine obese men; adipose biopsy after 72-hour fast.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transcript level is not direct PDE3B enzyme activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"A molecular brake on fat release changed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Substrate Metabolism and Insulin Sensitivity During Fasting in Obese Human Subjects: Impact of GH Blockade. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28324055/ · DOI 10.1210/jc.2016-3835","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0111521f-cdbc-531c-88fe-d93a098d5d72","evidence_kind":"source_excerpt","locator":"Lines 224-230","start_line":224,"end_line":230,"excerpt":"## fast-adipose-pde3b\nA molecular brake on fat release changed.\nAdipose PDE3B transcript levels decreased after fasting, with and without GH blockade.\nModel: Nine obese men; adipose biopsy after 72-hour fast.\nLimitations: Transcript level is not direct PDE3B enzyme activity.\nEvidence access: Primary full-text Results: Regulation of lipolysis, DOI 10.1210/jc.2016-3835\nSubstrate Metabolism and Insulin Sensitivity During Fasting in Obese Human Subjects: Impact of GH Blockade. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28324055/ · DOI 10.1210/jc.2016-3835","model_system":"Nine obese men; adipose biopsy after 72-hour fast.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"921d1eb5-2c88-52b9-9898-60b2b5bf6029","stable_key":"import-c9aa15a1-8ba2-5913-b2dd-372af304bf87","title":"Fasting: fuel switching, nutrient sensing, ketone signaling, nutrient dependencies and refeeding (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"991b7fd94be84c0f1e622e0672eda03d600b388dfd7825e219aa1678d1c7983a","revision_id":"ce85d3f4-4eaf-5aee-bddb-0f0ce7fe2211","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}