{"id":"59a0063f-9d0b-599c-9a63-703c4f8b4b41","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-kynu-plp","predicate":"cofactor-for","statement":"Human kynureninase crystallography resolves the PLP-containing active-site architecture.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"af0da268-b1eb-53a8-8b51-5aba3ed00347","mechanism_event_label":"KYNU uses active B6 in the tryptophan pathway.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"573bd034-6b1a-585e-9421-25ca28510d89","slug":"kynu","display_name":"Human kynureninase / KYNU","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"af0da268-b1eb-53a8-8b51-5aba3ed00347","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-kynu-plp-event","event_type":"biochemical_relationship","label":"KYNU uses active B6 in the tryptophan pathway.","description":"Human kynureninase crystallography resolves the PLP-containing active-site architecture.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"573bd034-6b1a-585e-9421-25ca28510d89","slug":"kynu","display_name":"Human kynureninase / KYNU","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B6-dependent KYNU acts in the pathway leading from tryptophan toward niacin/NAD precursors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant human KYNU crystallography and biochemical characterization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme evidence does not define dietary requirements or cellular PLP thresholds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"KYNU uses active B6 in the tryptophan pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-kynu-2007] Crystal Structure of Homo Sapiens Kynureninase (2007). https://pmc.ncbi.nlm.nih.gov/articles/PMC2531291/ DOI: 10.1021/bi0616697","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bbef75ee-3c81-59fe-ad32-ecb7e9d602f8","evidence_kind":"source_excerpt","locator":"Lines 873-883","start_line":873,"end_line":883,"excerpt":"### b6-met-kynu-plp\nHuman kynureninase crystallography resolves the PLP-containing active-site architecture.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: KYNU uses active B6 in the tryptophan pathway.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Recombinant human KYNU crystallography and biochemical characterization\nlimitations: Purified-enzyme evidence does not define dietary requirements or cellular PLP thresholds.\ncross_nutrient: B6-dependent KYNU acts in the pathway leading from tryptophan toward niacin/NAD precursors.\n[b6-kynu-2007] Crystal Structure of Homo Sapiens Kynureninase (2007). https://pmc.ncbi.nlm.nih.gov/articles/PMC2531291/ DOI: 10.1021/bi0616697","model_system":"Recombinant human KYNU crystallography and biochemical characterization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-kynu-2007] Crystal Structure of Homo Sapiens Kynureninase (2007). https://pmc.ncbi.nlm.nih.gov/articles/PMC2531291/ DOI: 10.1021/bi0616697","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}