{"id":"597a469b-6754-5687-bc61-e1c5858c8aac","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyatl1-isovaleryl","predicate":"forms_tested","statement":"Purified GLYATL1 also supported N-isovalerylglycine formation in vitro, less favorably than its preferred substrate reaction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6376aa19-472c-55f7-aa41-14409b45ed09","mechanism_event_label":"A related enzyme provides another possible route to the same product.","subject":{"id":"12170152-70f0-54b8-9ca7-3ed6dd9b8e7f","slug":"glyatl1","display_name":"Human glycine N-acyltransferase-like 1 / GLYATL1","entity_type_key":"protein"},"object":{"id":"3ac8c617-7037-540d-8eca-ce442f2036c2","slug":"isovalerylglycine","display_name":"N-Isovalerylglycine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6376aa19-472c-55f7-aa41-14409b45ed09","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyatl1-isovaleryl-event","event_type":"observed_relationship","label":"A related enzyme provides another possible route to the same product.","description":"Purified GLYATL1 also supported N-isovalerylglycine formation in vitro, less favorably than its preferred substrate reaction.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"12170152-70f0-54b8-9ca7-3ed6dd9b8e7f","slug":"glyatl1","display_name":"Human glycine N-acyltransferase-like 1 / GLYATL1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3ac8c617-7037-540d-8eca-ce442f2036c2","slug":"isovalerylglycine","display_name":"N-Isovalerylglycine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bb6288c5-83e5-5cbc-9e5f-b6c74eed8b8f","slug":"isovaleryl-coa","display_name":"Isovaleryl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified enzyme assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish which enzyme dominates in a patient.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A related enzyme provides another possible route to the same product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The glycine N-acyltransferases, GLYAT and GLYATL1, contribute to the detoxification of isovaleryl-CoA - an in-silico and in vitro validation. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36817957/ · DOI 10.1016/j.csbj.2023.01.041","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1c3957dc-a97c-5543-ba7d-1696d1e9333d","evidence_kind":"source_excerpt","locator":"Lines 298-304","start_line":298,"end_line":304,"excerpt":"## glycine-glyatl1-isovaleryl\nA related enzyme provides another possible route to the same product.\nPurified GLYATL1 also supported N-isovalerylglycine formation in vitro, less favorably than its preferred substrate reaction.\nModel: Purified enzyme assays.\nLimitations: Does not establish which enzyme dominates in a patient.\nEvidence access: Primary full text\nThe glycine N-acyltransferases, GLYAT and GLYATL1, contribute to the detoxification of isovaleryl-CoA - an in-silico and in vitro validation. · 2023 · https://pubmed.ncbi.nlm.nih.gov/36817957/ · DOI 10.1016/j.csbj.2023.01.041","model_system":"Purified enzyme assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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