{"id":"5915a53f-5418-530d-a0e3-c427426654e1","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-epac-nad-loss","predicate":"silencing_blocks","statement":"Epac1 siRNA prevented the resveratrol-induced NAD increase and PGC-1alpha deacetylation in human HeLa experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b50faa38-44ec-5a21-8b13-9570d1dbf92d","mechanism_event_label":"Calcium/cAMP signaling connects to NAD-dependent regulation.","subject":{"id":"eb3c3d8f-61e0-5daf-82ea-7906d7ae4f00","slug":"rapgef3","display_name":"Human Epac1 / RAPGEF3","entity_type_key":"protein"},"object":{"id":"1e6173c3-2364-57f4-bf4d-730ad5788944","slug":"human-hela-resveratrol-nad-response","display_name":"Resveratrol-associated NAD increase in HeLa cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b50faa38-44ec-5a21-8b13-9570d1dbf92d","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-epac-nad-loss-event","event_type":"observed_relationship","label":"Calcium/cAMP signaling connects to NAD-dependent regulation.","description":"Epac1 siRNA prevented the resveratrol-induced NAD increase and PGC-1alpha deacetylation in human HeLa experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eb3c3d8f-61e0-5daf-82ea-7906d7ae4f00","slug":"rapgef3","display_name":"Human Epac1 / RAPGEF3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1e6173c3-2364-57f4-bf4d-730ad5788944","slug":"human-hela-resveratrol-nad-response","display_name":"Resveratrol-associated NAD increase in HeLa cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b8e1bfa9-c412-5f35-97af-cfc722123d27","slug":"sirt1","display_name":"SIRT1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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