{"id":"58cc0896-6f1f-57b1-be4d-2c89042a3546","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-microbiota-transfer","predicate":"loss_alters_transmissible_microbiota","statement":"Microbiota transfer from Ace2-mutant mice transmitted increased colitis susceptibility to germ-free wild-type recipients.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"999d6a5c-e097-5577-97c8-5b264b1a27b9","mechanism_event_label":"The altered microbial community carried part of the susceptibility.","subject":{"id":"51b5a82d-32e8-5223-b384-026dd7cead16","slug":"mouse-ace2","display_name":"Mouse angiotensin-converting enzyme 2 / Ace2","entity_type_key":"protein"},"object":{"id":"8cb2bb35-537a-5db0-b138-94506b1303d1","slug":"mouse-trp-colitis-susceptibility","display_name":"Mouse susceptibility to chemically induced colitis in tryptophan experiments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"999d6a5c-e097-5577-97c8-5b264b1a27b9","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-microbiota-transfer-event","event_type":"observed_relationship","label":"The altered microbial community carried part of the susceptibility.","description":"Microbiota transfer from Ace2-mutant mice transmitted increased colitis susceptibility to germ-free wild-type recipients.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"51b5a82d-32e8-5223-b384-026dd7cead16","slug":"mouse-ace2","display_name":"Mouse angiotensin-converting enzyme 2 / Ace2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8cb2bb35-537a-5db0-b138-94506b1303d1","slug":"mouse-trp-colitis-susceptibility","display_name":"Mouse susceptibility to chemically induced colitis in tryptophan experiments","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eba9a619-26fc-540e-9a2d-6915c0b37e3a","slug":"mouse-trp-antimicrobial-peptides","display_name":"Mouse intestinal antimicrobial peptide expression in tryptophan experiments","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, microbiota-transfer results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse donor-to-recipient microbiota transfer.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transfer does not isolate one bacterial species or one metabolite.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The altered microbial community carried part of the susceptibility.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22837003/ · DOI 10.1038/nature11228","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9c64f25c-140c-568f-9a2b-718075d31bc4","evidence_kind":"source_excerpt","locator":"Lines 106-112","start_line":106,"end_line":112,"excerpt":"## tryptophan-microbiota-transfer\nThe altered microbial community carried part of the susceptibility.\nMicrobiota transfer from Ace2-mutant mice transmitted increased colitis susceptibility to germ-free wild-type recipients.\nModel: Mouse donor-to-recipient microbiota transfer.\nLimitations: Transfer does not isolate one bacterial species or one metabolite.\nEvidence access: Primary full text, microbiota-transfer results\nACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22837003/ · DOI 10.1038/nature11228","model_system":"Mouse donor-to-recipient microbiota transfer.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}