{"id":"58997fac-2fec-5aff-8061-09cd0f642225","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-lps-signal","predicate":"reduces_stimulated_reporter","statement":"MLG formulations reduced the LPS-induced reporter response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"79a659d4-c442-53e8-8114-a2fdf2a13554","mechanism_event_label":"The mixtures changed the response to bacterial endotoxin.","subject":{"id":"1cf59812-4833-503e-8665-091c254c34bb","slug":"mlg-50-fulvic-formulation","display_name":"MLG-50 fossil-derived fulvic/mineral formulation","entity_type_key":"chemical_species"},"object":{"id":"3d9f7705-e0ab-53df-9f8c-fc8d708f221a","slug":"human-thp1-nfkb-fa","display_name":"LPS-induced NF-kappa-B reporter response in human THP1-Blue cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"79a659d4-c442-53e8-8114-a2fdf2a13554","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-lps-signal-event","event_type":"observed_relationship","label":"The mixtures changed the response to bacterial endotoxin.","description":"MLG formulations reduced the LPS-induced reporter response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1cf59812-4833-503e-8665-091c254c34bb","slug":"mlg-50-fulvic-formulation","display_name":"MLG-50 fossil-derived fulvic/mineral formulation","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3d9f7705-e0ab-53df-9f8c-fc8d708f221a","slug":"human-thp1-nfkb-fa","display_name":"LPS-induced NF-kappa-B reporter response in human THP1-Blue cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"eb6cc922-7af3-5247-91b1-3354be3141bb","slug":"mlg-a50-fulvic-formulation","display_name":"MLG-A50 alkaline fulvic/mineral formulation","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e96aad69-74fb-55c6-8378-7d5c281003eb","slug":"lipopolysaccharide","display_name":"Lipopolysaccharide","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"2026 MLG-50/MLG-A50 study; fossil extracts contain fulvic fractions plus mineral/polyphenol components, stock pH about 1.97/12.1. THP1-Blue cells; 1:8192 dilution, LPS 10 or 50 ng/mL, 24 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Co-components prevent assigning an effect to a single fulvic molecule. In-vitro activity is not established clinical benefit; preparation and dilution remain essential. LPS interaction and receptor interference are proposed from functional assays; direct TLR4 binding is not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The mixtures changed the response to bacterial endotoxin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Integrated safety and microbiota profiling of fulvic acid formulations across in vitro and in vivo models. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41580488/ · DOI 10.1038/s41598-026-37331-2","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c5886fdf-f943-5b22-8665-291edb53b5d5","evidence_kind":"source_excerpt","locator":"Lines 236-242","start_line":236,"end_line":242,"excerpt":"## fulvic-acid-lps-signal\nThe mixtures changed the response to bacterial endotoxin.\nMLG formulations reduced the LPS-induced reporter response.\nModel: 2026 MLG-50/MLG-A50 study; fossil extracts contain fulvic fractions plus mineral/polyphenol components, stock pH about 1.97/12.1. THP1-Blue cells; 1:8192 dilution, LPS 10 or 50 ng/mL, 24 hours.\nLimitations: Co-components prevent assigning an effect to a single fulvic molecule. In-vitro activity is not established clinical benefit; preparation and dilution remain essential. LPS interaction and receptor interference are proposed from functional assays; direct TLR4 binding is not established.\nEvidence access: Primary full text\nIntegrated safety and microbiota profiling of fulvic acid formulations across in vitro and in vivo models. · 2026 · https://pubmed.ncbi.nlm.nih.gov/41580488/ · DOI 10.1038/s41598-026-37331-2","model_system":"2026 MLG-50/MLG-A50 study; fossil extracts contain fulvic fractions plus mineral/polyphenol components, stock pH about 1.97/12.1. THP1-Blue cells; 1:8192 dilution, LPS 10 or 50 ng/mL, 24 hours.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"52c2c3ee6bb23d943a40e1c39dd9de5cca7acd6efa72479cb824149e85cc7968","revision_id":"94dfc01b-c93e-55ff-8f48-bb2e9a1979f6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}