{"id":"5893d838-fee1-58bd-b5d1-6667dd293eec","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:fsl-p65","predicate":"context_dependent_observation","statement":"ALCAR increased acetylated p65 in Flinders Sensitive Line rats, without the same increase in control FRL rats.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"caad8537-3f3e-5ae2-923d-f0e984c185b7","mechanism_event_label":"ALCAR increased acetylated p65 in Flinders Sensitive Line rats, without the same increase in control FRL rats.","subject":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"object":{"id":"6bbf1b55-6364-58a2-b2bf-6d6d838f4fb9","slug":"rat-fsl-p65-k310-acetylation","display_name":"Rat FSL hippocampal/prefrontal p65 K310 acetylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"caad8537-3f3e-5ae2-923d-f0e984c185b7","stable_key":"e3f6ce02-0bd7-5103-acc1-68503ab6751b:fsl-p65-event","event_type":"experimental_observation","label":"ALCAR increased acetylated p65 in Flinders Sensitive Line rats, without the same increase in control FRL rats.","description":"### fsl-p65\n\nALCAR increased acetylated p65 in Flinders Sensitive Line rats, without the same increase in control FRL rats.\nH3K27 promoter enrichment and p65 acetylation are distinct measurements. The study did not trace administered ALCAR acetyl atoms into these sites.\nModel and exposure: 100 mg/kg intraperitoneal daily; 21-day biochemical comparison; Fig. 3C, anti-acetyl-K310-p65 assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: L-acetylcarnitine causes rapid antidepressant effects through the epigenetic induction of mGlu2 receptors. | 2013 | https://pubmed.ncbi.nlm.nih.gov/23382250/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC3607061/ | https://doi.org/10.1073/pnas.1216100110 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5ce394a9-6ec6-56dd-866f-37d85c0556e4","slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"ALCAR in FSL and FRL animals","sequence_order":0,"notes":""},{"entity":{"id":"6bbf1b55-6364-58a2-b2bf-6d6d838f4fb9","slug":"rat-fsl-p65-k310-acetylation","display_name":"Rat FSL hippocampal/prefrontal p65 K310 acetylation","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"mixed","sequence_order":1,"notes":""},{"entity":{"id":"f6a4422d-82bc-5979-b1d9-54984971b126","slug":"rat-rela","display_name":"Rat NF-kappaB p65 / Rela","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1dd1a4e1-dc80-5297-8b76-98652b5150ce","slug":"rat-grm2","display_name":"Rat metabotropic glutamate receptor 2 / Grm2","entity_type_key":"protein"},"role":"downstream expression context","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"ALCAR in FSL and FRL animals\", \"comparator\": \"Corresponding saline-treated strain\", \"endpoint\": \"ALCAR increased acetylated p65 in Flinders Sensitive Line rats, without the same increase in control FRL rats.\", \"effect_direction\": \"mixed\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"100 mg/kg intraperitoneal daily; 21-day biochemical comparison; Fig. 3C, anti-acetyl-K310-p65 assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived curation with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Acetyl-L-carnitine collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"acetylcarnitine","display_name":"Acetyl-L-carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"ALCAR increased acetylated p65 in Flinders Sensitive Line rats, without the same increase in control FRL rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"L-acetylcarnitine causes rapid antidepressant effects through the epigenetic induction of mGlu2 receptors. | https://pubmed.ncbi.nlm.nih.gov/23382250/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC3607061/ | https://doi.org/10.1073/pnas.1216100110","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference fsl-p65; H3K27 promoter enrichment and p65 acetylation are distinct measurements. The study did not trace administered ALCAR acetyl atoms into these sites.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ab54a979-5ee5-5e69-a362-904d2a62f8a0","evidence_kind":"source_excerpt","locator":"Lines 224-230","start_line":224,"end_line":230,"excerpt":"### fsl-p65\n\nALCAR increased acetylated p65 in Flinders Sensitive Line rats, without the same increase in control FRL rats.\nH3K27 promoter enrichment and p65 acetylation are distinct measurements. The study did not trace administered ALCAR acetyl atoms into these sites.\nModel and exposure: 100 mg/kg intraperitoneal daily; 21-day biochemical comparison; Fig. 3C, anti-acetyl-K310-p65 assay.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: L-acetylcarnitine causes rapid antidepressant effects through the epigenetic induction of mGlu2 receptors. | 2013 | https://pubmed.ncbi.nlm.nih.gov/23382250/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC3607061/ | https://doi.org/10.1073/pnas.1216100110 | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"100 mg/kg intraperitoneal daily; 21-day biochemical comparison; Fig. 3C, anti-acetyl-K310-p65 assay.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2588f904-245a-597a-8a12-65118013fb90","stable_key":"import-e3f6ce02-0bd7-5103-acc1-68503ab6751b","title":"Acetyl-L-carnitine: detailed mechanisms of action (reviewed 6 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original review of primary studies and clearly identified official records. Exact primary access is stated; no independent replication is claimed.","file_path":"","sha256":"84cc82d6fbb0fd5d73c4ce779e9f88e7f0184ba2d29b18ad0f153815380f2975","revision_id":"dac7a950-dbe5-5c7a-9c70-2ea711d5c71a","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}