{"id":"582b4c44-4f6c-5f06-8ee5-a8a6a0eb488c","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-bone-treg-wnt","predicate":"modulates","statement":"Butyrate expanded gut and bone-marrow Tregs; their interaction with CD8 T cells increased Wnt10b production and bone formation in young mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d89274fd-bb67-5723-8056-0c54e342cb89","mechanism_event_label":"An immune-cell relay connected a gut metabolite to bone-building signals.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"e408fd51-36b5-565a-be99-a532b9aec9c7","slug":"mouse-butyrate-bone-formation","display_name":"Mouse bone formation after butyrate","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d89274fd-bb67-5723-8056-0c54e342cb89","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-bone-treg-wnt-event","event_type":"observed_relationship","label":"An immune-cell relay connected a gut metabolite to bone-building signals.","description":"Butyrate expanded gut and bone-marrow Tregs; their interaction with CD8 T cells increased Wnt10b production and bone formation in young mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e408fd51-36b5-565a-be99-a532b9aec9c7","slug":"mouse-butyrate-bone-formation","display_name":"Mouse bone formation after butyrate","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"24f2ec5a-b6dd-5e40-bd54-b5650827ef07","slug":"mouse-wnt10b","display_name":"Mouse Wnt family member 10B / Wnt10b","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"276ab0c0-74c4-5951-bf5f-32d85edb02db","slug":"mouse-foxp3","display_name":"Mouse regulatory T-cell transcription factor Foxp3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Young eugonadic mice; butyrate and separate probiotic interventions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No human fracture-prevention result; mineral substrate requirements were not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An immune-cell relay connected a gut metabolite to bone-building signals.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30446387/ · DOI 10.1016/j.immuni.2018.10.013","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"48f5491c-dfde-559d-9d28-91c92e66d4ee","evidence_kind":"source_excerpt","locator":"Lines 454-460","start_line":454,"end_line":460,"excerpt":"## butyrate-bone-treg-wnt\nAn immune-cell relay connected a gut metabolite to bone-building signals.\nButyrate expanded gut and bone-marrow Tregs; their interaction with CD8 T cells increased Wnt10b production and bone formation in young mice.\nModel: Young eugonadic mice; butyrate and separate probiotic interventions.\nLimitations: No human fracture-prevention result; mineral substrate requirements were not tested.\nEvidence access: Primary abstract\nThe Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. · 2018 · https://pubmed.ncbi.nlm.nih.gov/30446387/ · DOI 10.1016/j.immuni.2018.10.013","model_system":"Young eugonadic mice; butyrate and separate probiotic interventions.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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