{"id":"582231f7-870e-5fa1-8a1c-777943bff748","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-g6pd-expression","predicate":"increased_expression_of","statement":"Sulforaphane increased G6PD expression in the fibroblast study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bbd80575-0a0e-50da-987a-83cb027396d0","mechanism_event_label":"The response includes machinery connected to the cellular reducing-power supply.","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"f65184d1-6b79-5e38-8137-a06a40e94e36","slug":"g6pd","display_name":"Glucose-6-phosphate dehydrogenase / G6PD","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bbd80575-0a0e-50da-987a-83cb027396d0","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-g6pd-expression-event","event_type":"biochemical_relationship","label":"The response includes machinery connected to the cellular reducing-power supply.","description":"Sulforaphane increased G6PD expression in the fibroblast study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f65184d1-6b79-5e38-8137-a06a40e94e36","slug":"g6pd","display_name":"Glucose-6-phosphate dehydrogenase / G6PD","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/30595796.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"aa7d5e0ef15224906eb42eb49309439c966c30310740dbdea9553b76b2f91274\", \"start_char\": 0, \"end_char\": 2097, \"text_sha256\": \"aa7d5e0ef15224906eb42eb49309439c966c30310740dbdea9553b76b2f91274\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Replicative culture and metabolic/gene-expression measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane treatment during serial culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture senescence is not human longevity; expression is not necessarily flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human MRC-5 and BJ fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The response includes machinery connected to the cellular reducing-power supply.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p30595796] Sulforaphane Delays Fibroblast Senescence by Curbing Cellular Glucose Uptake, Increased Glycolysis, and Oxidative Damage. (2018). https://pubmed.ncbi.nlm.nih.gov/30595796/ DOI: 10.1155/2018/5642148","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Glucose handling, antioxidant response and senescence","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fda8a685-066e-56fb-8035-4f8e9025b2f0","evidence_kind":"source_excerpt","locator":"Lines 879-890","start_line":879,"end_line":890,"excerpt":"### sulforaphane-g6pd-expression\nSulforaphane increased G6PD expression in the fibroblast study.\nCondition category: normal\nnutrient_topic: Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The response includes machinery connected to the cellular reducing-power supply.\norganism: Human MRC-5 and BJ fibroblasts\ntissue_or_cell_type: Glucose handling, antioxidant response and senescence\nexperimental_model: Replicative culture and metabolic/gene-expression measurements\nlimitations: Cell-culture senescence is not human longevity; expression is not necessarily flux.\nexposure: Sulforaphane treatment during serial culture\nevidence_span: {\"source_cache\": \"artifacts/sulforaphane-research/30595796.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"aa7d5e0ef15224906eb42eb49309439c966c30310740dbdea9553b76b2f91274\", \"start_char\": 0, \"end_char\": 2097, \"text_sha256\": \"aa7d5e0ef15224906eb42eb49309439c966c30310740dbdea9553b76b2f91274\"}\n[sulforaphane-p30595796] Sulforaphane Delays Fibroblast Senescence by Curbing Cellular Glucose Uptake, Increased Glycolysis, and Oxidative Damage. 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