{"id":"5819ac92-139c-5f7d-bac3-c235453dc445","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-zebrafish-low","predicate":"reduces_contextual_signal","statement":"FulvoFeed at 5 or 50 mg C/L reduced the subsequent copper-associated ROS response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a6f91998-a56e-599e-844a-4cb56d6d8718","mechanism_event_label":"Lower tested exposures dampened the stress readout.","subject":{"id":"6ff82375-9f80-56e3-9b94-1d8288f61df7","slug":"fulvofeed-fulvic-2022","display_name":"FulvoFeed phenol-rich fulvic preparation, 2022 study","entity_type_key":"chemical_species"},"object":{"id":"d440af4c-827d-5a3b-b8b1-7c49d9a24369","slug":"zebrafish-copper-ros","display_name":"Copper-associated ROS fluorescence in zebrafish larvae","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a6f91998-a56e-599e-844a-4cb56d6d8718","stable_key":"788beb7b-7928-5f89-83d4-5556abf3702c:fulvic-acid-zebrafish-low-event","event_type":"observed_relationship","label":"Lower tested exposures dampened the stress readout.","description":"FulvoFeed at 5 or 50 mg C/L reduced the subsequent copper-associated ROS response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6ff82375-9f80-56e3-9b94-1d8288f61df7","slug":"fulvofeed-fulvic-2022","display_name":"FulvoFeed phenol-rich fulvic preparation, 2022 study","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d440af4c-827d-5a3b-b8b1-7c49d9a24369","slug":"zebrafish-copper-ros","display_name":"Copper-associated ROS fluorescence in zebrafish larvae","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0aa6c0fc-8374-56e0-b172-6c40b6366d21","slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Zebrafish larvae; FulvoFeed for 96 hours, wash, then copper 50 micromolar for 2 hours; ROS fluorescence assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"mg C/L expresses organic carbon, not supplement mass. Washing reduced direct external copper-complexation confounding. ROS is an indirect inflammatory endpoint; proposed radical/aromatic explanations were not isolated genetic or binding tests.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fulvic acid collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fulvic-acid","display_name":"Fulvic acid (heterogeneous humic fraction)","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Lower tested exposures dampened the stress readout.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Modification of the chemically induced inflammation assay reveals the Janus face of a phenol rich fulvic acid. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35393468/ · DOI 10.1038/s41598-022-09782-w","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"10cd837f-62d8-5551-aea4-83d693753511","evidence_kind":"source_excerpt","locator":"Lines 196-202","start_line":196,"end_line":202,"excerpt":"## fulvic-acid-zebrafish-low\nLower tested exposures dampened the stress readout.\nFulvoFeed at 5 or 50 mg C/L reduced the subsequent copper-associated ROS response.\nModel: Zebrafish larvae; FulvoFeed for 96 hours, wash, then copper 50 micromolar for 2 hours; ROS fluorescence assay.\nLimitations: mg C/L expresses organic carbon, not supplement mass. Washing reduced direct external copper-complexation confounding. ROS is an indirect inflammatory endpoint; proposed radical/aromatic explanations were not isolated genetic or binding tests.\nEvidence access: Primary full text\nModification of the chemically induced inflammation assay reveals the Janus face of a phenol rich fulvic acid. · 2022 · https://pubmed.ncbi.nlm.nih.gov/35393468/ · DOI 10.1038/s41598-022-09782-w","model_system":"Zebrafish larvae; FulvoFeed for 96 hours, wash, then copper 50 micromolar for 2 hours; ROS fluorescence assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b00a1818-46e4-52cc-8dfe-a647d5c08ed7","stable_key":"import-788beb7b-7928-5f89-83d4-5556abf3702c","title":"Fulvic acid: mixture identity, mineral chemistry, signaling and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"52c2c3ee6bb23d943a40e1c39dd9de5cca7acd6efa72479cb824149e85cc7968","revision_id":"94dfc01b-c93e-55ff-8f48-bb2e9a1979f6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}