{"id":"57ed7db1-cbb8-5841-98f1-3800e4af775e","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-arg2-hydrolysis","predicate":"hydrolyzes","statement":"Purified recombinant human ARG2 catalyzes arginine hydrolysis to ornithine and urea.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"54c16540-308c-5e68-9b1c-98d5af85adfa","mechanism_event_label":"ARG2 breaks arginine into ornithine and urea.","subject":{"id":"8ea15e8d-d518-5929-997d-0fa4091785fb","slug":"arg2","display_name":"Human arginase 2 / ARG2","entity_type_key":"protein"},"object":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"54c16540-308c-5e68-9b1c-98d5af85adfa","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-arg2-hydrolysis-event","event_type":"biochemical_relationship","label":"ARG2 breaks arginine into ornithine and urea.","description":"Purified recombinant human ARG2 catalyzes arginine hydrolysis to ornithine and urea.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ea15e8d-d518-5929-997d-0fa4091785fb","slug":"arg2","display_name":"Human arginase 2 / ARG2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c715e48e-42db-5fac-b4a4-c1285a68d085","slug":"ornithine","display_name":"L-Ornithine","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"37cf0152-db25-5773-a726-d0c0facb2e24","slug":"urea","display_name":"Urea","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Recombinant human ARG2 expressed in E. coli and purified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Substrate/product kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primary experimental scope only; no human dietary threshold, clinical treatment rule, or universal metal substitution is established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ARG2 breaks arginine into ornithine and urea.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-enz-11370664] Expression, purification, and characterization of human type II arginase. 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