{"id":"57e58236-806d-5b40-bf0f-40c7b9f05f68","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-c2c12-membrane-repair","predicate":"promotes","statement":"Loading C2C12 cells with 200 µM alpha-tocopherol for 18 hours reduced FM1-43 entry after laser injury in calcium-containing buffer, consistent with faster membrane resealing.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"acc23e16-f305-5e81-bdcc-c935cc608134","mechanism_event_label":"Vitamin E loading helped cultured muscle-line cells close laser-induced membrane wounds.","subject":{"id":"1e078aed-0416-5fe7-8b8b-36bc2ca8ab7a","slug":"all-rac-alpha-tocopherol","display_name":"All-rac-alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"19860ac9-fc80-51bb-9bee-33f193616b4c","slug":"plasma-membrane-repair","display_name":"Plasma membrane repair","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"acc23e16-f305-5e81-bdcc-c935cc608134","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-c2c12-membrane-repair-event","event_type":"biochemical_relationship","label":"Vitamin E loading helped cultured muscle-line cells close laser-induced membrane wounds.","description":"Loading C2C12 cells with 200 µM alpha-tocopherol for 18 hours reduced FM1-43 entry after laser injury in calcium-containing buffer, consistent with faster membrane resealing.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"extracellular repair condition","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f0b6bcb6-5d41-5d9d-af9f-6af1a94d5c5a","slug":"plasma-membrane","display_name":"Plasma membrane","entity_type_key":"organelle"},"role":"injured membrane","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1e078aed-0416-5fe7-8b8b-36bc2ca8ab7a","slug":"all-rac-alpha-tocopherol","display_name":"All-rac-alpha-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"19860ac9-fc80-51bb-9bee-33f193616b4c","slug":"plasma-membrane-repair","display_name":"Plasma membrane repair","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 1a–b","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Laser wounding and time-resolved dye exclusion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"200 µM racemic alpha-tocopherol (96% pure) for 18 h, washed; 1.2 mM extracellular calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacologic cell loading; not a dietary intake equivalent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin E loading helped cultured muscle-line cells close laser-induced membrane wounds.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-howard2011] Promotion of plasma membrane repair by vitamin E. (2011). https://pubmed.ncbi.nlm.nih.gov/22186893/ DOI: 10.1038/ncomms1594","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"C2C12 myoblasts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7f70fe3a-ed15-5f40-b430-3bb8a0ce0b9c","evidence_kind":"source_excerpt","locator":"Lines 662-674","start_line":662,"end_line":674,"excerpt":"### ver-c2c12-membrane-repair\nLoading C2C12 cells with 200 µM alpha-tocopherol for 18 hours reduced FM1-43 entry after laser injury in calcium-containing buffer, consistent with faster membrane resealing.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin E loading helped cultured muscle-line cells close laser-induced membrane wounds.\norganism: Mus musculus\ntissue_or_cell_type: C2C12 myoblasts\nexperimental_model: Laser wounding and time-resolved dye exclusion\nlimitations: Pharmacologic cell loading; not a dietary intake equivalent.\nexposure: 200 µM racemic alpha-tocopherol (96% pure) for 18 h, washed; 1.2 mM extracellular calcium.\ncross_nutrient: true\nevidence_location: Figure 1a–b\n[ver-howard2011] Promotion of plasma membrane repair by vitamin E. 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