{"id":"5762218a-edb7-531c-a2a5-bd5f4ca25b50","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-lias-embryonic-loss","predicate":"genetic_loss_reduces","statement":"Lias-null mouse embryos died before embryonic day 9.5.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"13385715-282d-5f2f-b115-ac96a628275b","mechanism_event_label":"The developing embryo required its own cofactor-synthesis machinery.","subject":{"id":"1f594137-0a3a-5424-84cb-50da9ff116cc","slug":"mouse-lias","display_name":"Mouse lipoyl synthase / Lias","entity_type_key":"protein"},"object":{"id":"215f7d2f-598e-5f85-9d99-8a6556917471","slug":"mouse-lias-null-embryonic-survival","display_name":"Survival of Lias-null mouse embryos","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"13385715-282d-5f2f-b115-ac96a628275b","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-lias-embryonic-loss-event","event_type":"biochemical_relationship","label":"The developing embryo required its own cofactor-synthesis machinery.","description":"Lias-null mouse embryos died before embryonic day 9.5.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1f594137-0a3a-5424-84cb-50da9ff116cc","slug":"mouse-lias","display_name":"Mouse lipoyl synthase / Lias","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"215f7d2f-598e-5f85-9d99-8a6556917471","slug":"mouse-lias-null-embryonic-survival","display_name":"Survival of Lias-null mouse embryos","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/16135825.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b1750e4eb7b27e8b28bf973a3683d88c6ad9d639acc10f3f4c5df6077fb384da\", \"start_char\": 0, \"end_char\": 1393, \"text_sha256\": \"b1750e4eb7b27e8b28bf973a3683d88c6ad9d639acc10f3f4c5df6077fb384da\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Targeted Lias knockout and maternal supplementation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lias-null embryos; high maternal lipoic-acid intake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Embryonic genetic loss is not a dietary depletion model in humans.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The developing embryo required its own cofactor-synthesis machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p16135825] Endogenous production of lipoic acid is essential for mouse development. (2005). https://pubmed.ncbi.nlm.nih.gov/16135825/ DOI: 10.1128/mcb.25.18.8387-8392.2005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Embryos and pregnant dams","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"09792bb7-8656-501e-93ff-74cc06f17d15","evidence_kind":"source_excerpt","locator":"Lines 585-596","start_line":585,"end_line":596,"excerpt":"### ala-lias-embryonic-loss\nLias-null mouse embryos died before embryonic day 9.5.\nCondition category: machinery_impairment\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The developing embryo required its own cofactor-synthesis machinery.\norganism: Mouse\ntissue_or_cell_type: Embryos and pregnant dams\nexperimental_model: Targeted Lias knockout and maternal supplementation\nlimitations: Embryonic genetic loss is not a dietary depletion model in humans.\nexposure: Lias-null embryos; high maternal lipoic-acid intake\nevidence_span: {\"source_cache\": \"artifacts/ala-research/16135825.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"b1750e4eb7b27e8b28bf973a3683d88c6ad9d639acc10f3f4c5df6077fb384da\", \"start_char\": 0, \"end_char\": 1393, \"text_sha256\": \"b1750e4eb7b27e8b28bf973a3683d88c6ad9d639acc10f3f4c5df6077fb384da\"}\n[ala-p16135825] Endogenous production of lipoic acid is essential for mouse development. (2005). https://pubmed.ncbi.nlm.nih.gov/16135825/ DOI: 10.1128/mcb.25.18.8387-8392.2005","model_system":"Targeted Lias knockout and maternal supplementation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ala-p16135825] Endogenous production of lipoic acid is essential for mouse development. (2005). https://pubmed.ncbi.nlm.nih.gov/16135825/ DOI: 10.1128/mcb.25.18.8387-8392.2005","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d8afa8c2-ced9-5b28-90ca-2ac120ec7202","stable_key":"import-5d8e27d8-6a74-5560-827f-3f90908bbc34","title":"Alpha-lipoic acid: cofactor assembly, redox signaling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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