{"id":"575e8620-eb1d-502b-a47c-d5c98e2be617","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-mouse-ampk-dose","predicate":"activates","statement":"In mouse C2C12 cells, 25 micromolar resveratrol activated AMPK in a Sirt1-dependent manner; at 50 micromolar, activation could occur independently of Sirt1.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"358cfa04-a73f-5c7d-b360-75aad6be193c","mechanism_event_label":"The route to the same signaling node changes with exposure.","subject":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"object":{"id":"871e74d6-6e9e-5e9c-9c6b-f269f59cb02a","slug":"mouse-ampk-complexes","display_name":"Mouse AMPK complexes","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"358cfa04-a73f-5c7d-b360-75aad6be193c","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-mouse-ampk-dose-event","event_type":"observed_relationship","label":"The route to the same signaling node changes with exposure.","description":"In mouse C2C12 cells, 25 micromolar resveratrol activated AMPK in a Sirt1-dependent manner; at 50 micromolar, activation could occur independently of Sirt1.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"871e74d6-6e9e-5e9c-9c6b-f269f59cb02a","slug":"mouse-ampk-complexes","display_name":"Mouse AMPK complexes","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dbe09a67-bc1f-53d9-b555-580c204b2bbb","slug":"mouse-sirt1","display_name":"Mouse sirtuin 1 / Sirt1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse muscle-cell dose comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Neither concentration is a demonstrated human muscle exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The route to the same signaling node changes with exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560220/ · DOI 10.1016/j.cmet.2012.04.003","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0e5324b6-0916-5ab3-b511-c5ee302fe692","evidence_kind":"source_excerpt","locator":"Lines 182-188","start_line":182,"end_line":188,"excerpt":"## resveratrol-mouse-ampk-dose\nThe route to the same signaling node changes with exposure.\nIn mouse C2C12 cells, 25 micromolar resveratrol activated AMPK in a Sirt1-dependent manner; at 50 micromolar, activation could occur independently of Sirt1.\nModel: Mouse muscle-cell dose comparison.\nLimitations: Neither concentration is a demonstrated human muscle exposure.\nEvidence access: Primary full text\nSIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22560220/ · DOI 10.1016/j.cmet.2012.04.003","model_system":"Mouse muscle-cell dose comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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