{"id":"5733d291-11c2-5f6e-a19b-ffc8835d5be9","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-oxidant-challenged-repair","predicate":"preserves","statement":"Pretreatment of BS-C-1 cells with 200 µM alpha-tocopherol for 24 hours prevented the membrane-repair defect caused by 1 mM hydrogen peroxide during laser injury.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"29e2ff6f-2e46-575e-b8a9-3c3864220d38","mechanism_event_label":"Vitamin E loading protected the repair response against this oxidant challenge.","subject":{"id":"1e078aed-0416-5fe7-8b8b-36bc2ca8ab7a","slug":"all-rac-alpha-tocopherol","display_name":"All-rac-alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"19860ac9-fc80-51bb-9bee-33f193616b4c","slug":"plasma-membrane-repair","display_name":"Plasma membrane repair","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"29e2ff6f-2e46-575e-b8a9-3c3864220d38","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-oxidant-challenged-repair-event","event_type":"biochemical_relationship","label":"Vitamin E loading protected the repair response against this oxidant challenge.","description":"Pretreatment of BS-C-1 cells with 200 µM alpha-tocopherol for 24 hours prevented the membrane-repair defect caused by 1 mM hydrogen peroxide during laser injury.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"oxidant challenge","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1e078aed-0416-5fe7-8b8b-36bc2ca8ab7a","slug":"all-rac-alpha-tocopherol","display_name":"All-rac-alpha-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"19860ac9-fc80-51bb-9bee-33f193616b4c","slug":"plasma-membrane-repair","display_name":"Plasma membrane repair","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 4a–b","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Oxidant challenge during laser-wound assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"200 µM racemic alpha-tocopherol (96% pure), 24 h; 1 mM H2O2 during injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Oxidant and loading concentrations are experimental; no specific fusion protein target was identified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"African green monkey-derived cell line","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin E loading protected the repair response against this oxidant challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-howard2011] Promotion of plasma membrane repair by vitamin E. (2011). https://pubmed.ncbi.nlm.nih.gov/22186893/ DOI: 10.1038/ncomms1594","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"BS-C-1 kidney epithelial cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c7f77a5d-885e-5bb2-8536-1b58c4c74fe9","evidence_kind":"source_excerpt","locator":"Lines 690-702","start_line":690,"end_line":702,"excerpt":"### ver-oxidant-challenged-repair\nPretreatment of BS-C-1 cells with 200 µM alpha-tocopherol for 24 hours prevented the membrane-repair defect caused by 1 mM hydrogen peroxide during laser injury.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin E loading protected the repair response against this oxidant challenge.\norganism: African green monkey-derived cell line\ntissue_or_cell_type: BS-C-1 kidney epithelial cells\nexperimental_model: Oxidant challenge during laser-wound assay\nlimitations: Oxidant and loading concentrations are experimental; no specific fusion protein target was identified.\nexposure: 200 µM racemic alpha-tocopherol (96% pure), 24 h; 1 mM H2O2 during injury.\ncross_nutrient: false\nevidence_location: Figure 4a–b\n[ver-howard2011] Promotion of plasma membrane repair by vitamin E. 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