{"id":"57300c79-a8d9-5970-b40a-4468782ff949","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-syn-tpo-protein-iodination","predicate":"catalyzes","statement":"Purified hog TPO catalyzed iodine incorporation into hog thyroglobulin in kinetic assays of protein iodination.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"48d8356f-179d-59a2-b3ea-d7932b24f864","mechanism_event_label":"TPO attaches iodine to tyrosine residues within the TG protein.","subject":{"id":"a524c063-3d56-5ec2-95c1-6bb74b14af82","slug":"porcine-tpo","display_name":"Porcine thyroid peroxidase","entity_type_key":"protein"},"object":{"id":"9a04d3c7-64ad-58cb-87f1-33b284599148","slug":"porcine-thyroglobulin-iodination","display_name":"Porcine thyroglobulin iodination","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"48d8356f-179d-59a2-b3ea-d7932b24f864","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-syn-tpo-protein-iodination-event","event_type":"biochemical_relationship","label":"TPO attaches iodine to tyrosine residues within the TG protein.","description":"Purified hog TPO catalyzed iodine incorporation into hog thyroglobulin in kinetic assays of protein iodination.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"74b9436f-3eb4-5e91-8981-f72f13c0feba","slug":"porcine-thyroglobulin","display_name":"Porcine thyroglobulin","entity_type_key":"protein"},"role":"protein_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"iodine_source","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"da9d64bc-69d4-5d97-90a4-8f0ed03e0ac8","slug":"hydrogen-peroxide","display_name":"Hydrogen peroxide","entity_type_key":"small_molecule"},"role":"peroxidase_reaction_partner","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fd936be6-d715-5f97-a13e-3221b5edac60","slug":"tg-bound-monoiodotyrosyl-residues","display_name":"Thyroglobulin-bound monoiodotyrosyl residues","entity_type_key":"chemical_species"},"role":"protein_bound_iodination_form","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ca877817-8e7f-5da4-b3c5-2a6345ddfae0","slug":"tg-bound-diiodotyrosyl-residues","display_name":"Thyroglobulin-bound diiodotyrosyl residues","entity_type_key":"chemical_species"},"role":"protein_bound_iodination_form","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"a524c063-3d56-5ec2-95c1-6bb74b14af82","slug":"porcine-tpo","display_name":"Porcine thyroid peroxidase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"9a04d3c7-64ad-58cb-87f1-33b284599148","slug":"porcine-thyroglobulin-iodination","display_name":"Porcine thyroglobulin iodination","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iodine-synthesis-sources/6706940.json\", \"json_field\": \"abstractText\", \"text_sha256\": \"e7d184d64b66b0dc89f61c511688c79ffc9785b20233e7746ef4c97de351c78c\", \"text_characters\": 1442, \"note\": \"Exact publisher passage retained in the cited local source cache; locator and digest supplied here.\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified hog thyroid peroxidase with hog thyroglobulin; steady-state and stopped-flow kinetics","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified hog TPO; native TG with 1.0% iodine and further-iodinated TG with 1.2% iodine; reagent concentrations not stated in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reaction measures TG iodination, not free iodotyrosine recycling. The proposed iodinium intermediate is a model, not imported as an established mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Sus scrofa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"TPO attaches iodine to tyrosine residues within the TG protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-syn-tpo-iodination1984] Iodination and oxidation of thyroglobulin catalyzed by thyroid peroxidase. (1984). https://pubmed.ncbi.nlm.nih.gov/6706940/ DOI: 10.1016/s0021-9258(17)43667-2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified thyroid enzyme and protein substrate","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eee3873d-0e66-5dea-a0d5-a5251c326a80","evidence_kind":"source_excerpt","locator":"Lines 828-840","start_line":828,"end_line":840,"excerpt":"### iodine-syn-tpo-protein-iodination\nPurified hog TPO catalyzed iodine incorporation into hog thyroglobulin in kinetic assays of protein iodination.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: TPO attaches iodine to tyrosine residues within the TG protein.\norganism: Sus scrofa\ntissue_or_cell_type: Purified thyroid enzyme and protein substrate\nexperimental_model: Purified hog thyroid peroxidase with hog thyroglobulin; steady-state and stopped-flow kinetics\nlimitations: Reaction measures TG iodination, not free iodotyrosine recycling. The proposed iodinium intermediate is a model, not imported as an established mechanism.\nexposure: Purified hog TPO; native TG with 1.0% iodine and further-iodinated TG with 1.2% iodine; reagent concentrations not stated in abstract.\ncross_nutrient: false\nevidence_span: {\"source_cache\": \"artifacts/iodine-synthesis-sources/6706940.json\", \"json_field\": \"abstractText\", \"text_sha256\": \"e7d184d64b66b0dc89f61c511688c79ffc9785b20233e7746ef4c97de351c78c\", \"text_characters\": 1442, \"note\": \"Exact publisher passage retained in the cited local source cache; locator and digest supplied here.\"}\n[iodine-syn-tpo-iodination1984] Iodination and oxidation of thyroglobulin catalyzed by thyroid peroxidase. (1984). https://pubmed.ncbi.nlm.nih.gov/6706940/ DOI: 10.1016/s0021-9258(17)43667-2","model_system":"Purified hog thyroid peroxidase with hog thyroglobulin; steady-state and stopped-flow kinetics","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-syn-tpo-iodination1984] Iodination and oxidation of thyroglobulin catalyzed by thyroid peroxidase. (1984). https://pubmed.ncbi.nlm.nih.gov/6706940/ DOI: 10.1016/s0021-9258(17)43667-2","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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