{"id":"572e4a35-8150-57f7-81dc-d5270644f116","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-mcu-matrix-uptake","predicate":"transports","statement":"MCU supplies the inner-mitochondrial-membrane calcium-conducting pore and promotes agonist-evoked matrix calcium uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"3e3cef26-f9c7-5a30-9e7e-1568c6a7f34d","mechanism_event_label":"MCU lets calcium enter the mitochondrial matrix.","subject":{"id":"8327f938-e484-5212-9580-7446b5c68ad8","slug":"mcu","display_name":"Mitochondrial calcium uniporter pore protein / MCU","entity_type_key":"protein"},"object":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"3e3cef26-f9c7-5a30-9e7e-1568c6a7f34d","stable_key":"434e1e27-eb90-583a-956b-62472726ffba:ca-mcu-matrix-uptake-event","event_type":"transport","label":"MCU lets calcium enter the mitochondrial matrix.","description":"MCU supplies the inner-mitochondrial-membrane calcium-conducting pore and promotes agonist-evoked matrix calcium uptake.","status":"provisional","compartment":{"slug":"inner-mitochondrial-membrane","display_name":"Inner mitochondrial membrane"},"participants":[{"entity":{"id":"8327f938-e484-5212-9580-7446b5c68ad8","slug":"mcu","display_name":"Mitochondrial calcium uniporter pore protein / MCU","entity_type_key":"protein"},"role":"channel","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9b7db5d3-7846-5363-b315-9ff69867f382","slug":"mitochondrial-calcium-uptake","display_name":"Mitochondrial calcium uptake","entity_type_key":"cellular_process"},"role":"process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4896b3f4-07bf-56d2-a369-c421f8b37ace","slug":"inner-mitochondrial-membrane","display_name":"Inner mitochondrial membrane","entity_type_key":"organelle"},"role":"experimental_location","stoichiometry":null,"state_label":"","sequence_order":3,"notes":"Study location; presence here is not a separate transport or causal claim."}]},"contexts":[{"dimension":"compartment_description","value_text":"Inner mitochondrial membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HeLa MCU silencing/overexpression and purified MCU in planar bilayers","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pore and overexpression evidence; native uptake also depends on accessory subunits and membrane potential.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Calcium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MCU lets calcium enter the mitochondrial matrix.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ca-destefani2011] A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter (2011). https://pubmed.ncbi.nlm.nih.gov/21685888/ DOI: 10.1038/nature10230","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"research_relationship_category","value_text":"transport","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HeLa cells and purified channel","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Recorded as promoting agonist-evoked matrix calcium uptake.","entity":null},{"dimension":"transport_or_reaction_direction","value_text":"Intermembrane space to mitochondrial matrix","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_pool","value_text":"mitochondrial matrix calcium","comparator":null,"unit":null,"notes":"Recorded as promoting agonist-evoked matrix calcium uptake.","entity":null}],"evidence":[{"id":"16045e9d-f351-5eaf-ac43-84f3bb35969e","evidence_kind":"source_excerpt","locator":"Lines 679-691","start_line":679,"end_line":691,"excerpt":"### ca-mcu-matrix-uptake\nMCU supplies the inner-mitochondrial-membrane calcium-conducting pore and promotes agonist-evoked matrix calcium uptake.\nCondition category: normal\nnutrient_topic: Calcium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: MCU lets calcium enter the mitochondrial matrix.\norganism: Homo sapiens\ntissue_or_cell_type: HeLa cells and purified channel\nexperimental_model: Human HeLa MCU silencing/overexpression and purified MCU in planar bilayers\nlimitations: Pore and overexpression evidence; native uptake also depends on accessory subunits and membrane potential.\nresearch_relationship_category: transport\ntransport_or_reaction_direction: Intermembrane space to mitochondrial matrix\ncompartment_description: Inner mitochondrial membrane\n[ca-destefani2011] A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter (2011). https://pubmed.ncbi.nlm.nih.gov/21685888/ DOI: 10.1038/nature10230","model_system":"Human HeLa MCU silencing/overexpression and purified MCU in planar bilayers","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ca-destefani2011] A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter (2011). https://pubmed.ncbi.nlm.nih.gov/21685888/ DOI: 10.1038/nature10230","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3c083fa0-59c6-50e9-af42-678d0e3c1006","stable_key":"import-434e1e27-eb90-583a-956b-62472726ffba","title":"Calcium: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"965c934d6b147f2b62f8d45ce6d7a87681a600bfe5c46a585b4704889afd3fc1","revision_id":"543ec48b-4f70-5197-9d76-f5a3c443664c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}