{"id":"5728d6fe-bd95-5ee1-8119-a51ddfb3162b","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-effector-state-limit","predicate":"does_not_reduce","statement":"Day-8 LCMV effector cells showed no significant reduction in granzyme B, IFN-gamma, TNF or Ki67 during the tested five-hour dialyzed-serum restimulation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"8e94b2be-eaaf-5bea-a931-ca71935c45a4","mechanism_event_label":"Already-active cells did not show the same short-term dependence as resting memory cells.","subject":{"id":"6b21a0aa-4ad9-5c81-b574-8b4e3e9d2eee","slug":"mouse-effector-alanine-deprivation","display_name":"Low extracellular alanine during mouse effector-cell restimulation","entity_type_key":"cellular_process"},"object":{"id":"28681a1a-97d2-50d3-a8b3-a7d48739cb3e","slug":"mouse-effector-cytokine-output","display_name":"Measured effector outputs in day-8 mouse CD8 T cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8e94b2be-eaaf-5bea-a931-ca71935c45a4","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-effector-state-limit-event","event_type":"observed_relationship","label":"Already-active cells did not show the same short-term dependence as resting memory cells.","description":"Day-8 LCMV effector cells showed no significant reduction in granzyme B, IFN-gamma, TNF or Ki67 during the tested five-hour dialyzed-serum restimulation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6b21a0aa-4ad9-5c81-b574-8b4e3e9d2eee","slug":"mouse-effector-alanine-deprivation","display_name":"Low extracellular alanine during mouse effector-cell restimulation","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"28681a1a-97d2-50d3-a8b3-a7d48739cb3e","slug":"mouse-effector-cytokine-output","display_name":"Measured effector outputs in day-8 mouse CD8 T cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse P14/LCMV effector phase; ex vivo peptide restimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A five-hour null result does not establish independence at every duration or tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Already-active cells did not show the same short-term dependence as resting memory cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"T Cell Activation Depends on Extracellular Alanine. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31533027/ · DOI 10.1016/j.celrep.2019.08.034","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"85011f7c-6761-598e-a826-9f39b2384359","evidence_kind":"source_excerpt","locator":"Lines 128-134","start_line":128,"end_line":134,"excerpt":"## alanine-effector-state-limit\nAlready-active cells did not show the same short-term dependence as resting memory cells.\nDay-8 LCMV effector cells showed no significant reduction in granzyme B, IFN-gamma, TNF or Ki67 during the tested five-hour dialyzed-serum restimulation.\nModel: Mouse P14/LCMV effector phase; 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