{"id":"5714bf74-206d-5d2d-a714-b5f73c661b9d","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-thiocyanate-inhibits","predicate":"inhibits","statement":"Thiocyanate inhibited radioactive iodide uptake in CHO cells stably expressing human NIS.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7a13108d-159c-592d-a088-9dbfdddcf1e9","mechanism_event_label":"A competing anion reduced iodide entry through human NIS in a cell assay.","subject":{"id":"5d54e0a3-869f-5132-82c9-4c0ff47cd400","slug":"thiocyanate-ion","display_name":"Thiocyanate ion","entity_type_key":"ion"},"object":{"id":"759b6f24-098d-5b19-b891-a5ad49fade50","slug":"cellular-iodide-uptake","display_name":"Cellular iodide uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7a13108d-159c-592d-a088-9dbfdddcf1e9","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-thiocyanate-inhibits-event","event_type":"biochemical_relationship","label":"A competing anion reduced iodide entry through human NIS in a cell assay.","description":"Thiocyanate inhibited radioactive iodide uptake in CHO cells stably expressing human NIS.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0d051c22-c0d2-5c22-91ad-4ee6894dc7a0","slug":"slc5a5","display_name":"Human sodium/iodide symporter / SLC5A5","entity_type_key":"protein"},"role":"inhibited_transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"competed_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5d54e0a3-869f-5132-82c9-4c0ff47cd400","slug":"thiocyanate-ion","display_name":"Thiocyanate ion","entity_type_key":"ion"},"role":"competing_anion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"759b6f24-098d-5b19-b891-a5ad49fade50","slug":"cellular-iodide-uptake","display_name":"Cellular iodide uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"CHO cells stably expressing human NIS; individual and mixed anion exposures with 125I uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Concentration-response experiments with individual anions and 125I uptake; exact concentration series not reported in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In vitro potency is not a human dietary exposure threshold, whole-body iodine displacement claim or supplementation instruction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human NIS in hamster CHO cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A competing anion reduced iodide entry through human NIS in a cell assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-inhibitors2004] Relative potencies and additivity of perchlorate, thiocyanate, nitrate, and iodide on the inhibition of radioactive iodide uptake by the human sodium iodide symporter. (2004). https://pubmed.ncbi.nlm.nih.gov/15650353/ DOI: 10.1089/thy.2004.14.1012","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9d84967f-0bee-5d21-a9b4-d7a7454d968e","evidence_kind":"source_excerpt","locator":"Lines 414-425","start_line":414,"end_line":425,"excerpt":"### iodine-trans-thiocyanate-inhibits\nThiocyanate inhibited radioactive iodide uptake in CHO cells stably expressing human NIS.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A competing anion reduced iodide entry through human NIS in a cell assay.\norganism: Human NIS in hamster CHO cells\ntissue_or_cell_type: Cell plasma membrane\nexperimental_model: CHO cells stably expressing human NIS; individual and mixed anion exposures with 125I uptake\nlimitations: In vitro potency is not a human dietary exposure threshold, whole-body iodine displacement claim or supplementation instruction.\nexposure: Concentration-response experiments with individual anions and 125I uptake; exact concentration series not reported in abstract.\ncross_nutrient: false\n[iodine-trans-inhibitors2004] Relative potencies and additivity of perchlorate, thiocyanate, nitrate, and iodide on the inhibition of radioactive iodide uptake by the human sodium iodide symporter. (2004). https://pubmed.ncbi.nlm.nih.gov/15650353/ DOI: 10.1089/thy.2004.14.1012","model_system":"CHO cells stably expressing human NIS; individual and mixed anion exposures with 125I uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-trans-inhibitors2004] Relative potencies and additivity of perchlorate, thiocyanate, nitrate, and iodide on the inhibition of radioactive iodide uptake by the human sodium iodide symporter. (2004). https://pubmed.ncbi.nlm.nih.gov/15650353/ DOI: 10.1089/thy.2004.14.1012","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a4885eb6f58bad4a4514c958b63834619e57b10aa7f694aa97d0f65c7d36548f","revision_id":"ea8001a1-b576-5eda-a193-883c7ad59b73","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}