{"id":"56e9c9ed-a33b-55c5-8438-1f7c35607a3e","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-iron-tpo-deficiency","predicate":"deficiency_reduced","statement":"Iron-deficiency anemia independently reduced thyroid peroxidase activity after accounting for food restriction in the rat experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8cb3c710-2ddf-59a2-93f1-cafb4770672a","mechanism_event_label":"Iodine supply is only part of thyroid-hormone synthesis; its working enzyme also depends on iron-containing heme.","subject":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"object":{"id":"fac2a7ba-0841-5ef9-9e01-90bb4f54217f","slug":"thyroid-peroxidase-activity","display_name":"Thyroid peroxidase activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8cb3c710-2ddf-59a2-93f1-cafb4770672a","stable_key":"e13b03e1-a614-543a-ac1b-97df18cfe30d:iron-iron-tpo-deficiency-event","event_type":"biochemical_relationship","label":"Iodine supply is only part of thyroid-hormone synthesis; its working enzyme also depends on iron-containing heme.","description":"Iron-deficiency anemia independently reduced thyroid peroxidase activity after accounting for food restriction in the rat experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f3ad4630-fa19-58aa-a347-32a539bceaae","slug":"rat-tpo","display_name":"Rat thyroid peroxidase / Tpo","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2e1f7e0a-8b54-5ea3-9d34-01e7167f9097","slug":"heme","display_name":"Heme","entity_type_key":"small_molecule"},"role":"prosthetic_group","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0e0ac6d1-3336-506a-9f69-5838ca1500ed","slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"},"role":"linked_nutrient","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"89bcaf42-b4ab-5760-8c2e-44eace10cee0","slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"fac2a7ba-0841-5ef9-9e01-90bb4f54217f","slug":"thyroid-peroxidase-activity","display_name":"Thyroid peroxidase activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/iron-research/12097675.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"26b7a5ddb0985abdb2325c7ecd505c46f02604bd4d35f68ed0c9ad67113a148e\", \"start_char\": 0, \"end_char\": 1527, \"text_sha256\": \"26b7a5ddb0985abdb2325c7ecd505c46f02604bd4d35f68ed0c9ad67113a148e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Controlled iron-deficient diets with pair-fed controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Four weeks of graded iron restriction; separate food-restriction controls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Food restriction also reduced TPO; the iron effect was separately estimated. No human serum threshold or automatic iodine-treatment failure rule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iron research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iron","display_name":"Iron","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"84 male weanling rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Iodine supply is only part of thyroid-hormone synthesis; its working enzyme also depends on iron-containing heme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iron-p12097675] Iron deficiency anemia reduces thyroid peroxidase activity in rats. (2002). https://pubmed.ncbi.nlm.nih.gov/12097675/ DOI: 10.1093/jn/132.7.1951","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Thyroid and blood","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"c7b80566-7c56-5fdf-86ae-cbaf0630b2e5","evidence_kind":"source_excerpt","locator":"Lines 901-912","start_line":901,"end_line":912,"excerpt":"### iron-iron-tpo-deficiency\nIron-deficiency anemia independently reduced thyroid peroxidase activity after accounting for food restriction in the rat experiment.\nCondition category: nutrient_deficiency\nnutrient_topic: Iron research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Iodine supply is only part of thyroid-hormone synthesis; its working enzyme also depends on iron-containing heme.\norganism: 84 male weanling rats\ntissue_or_cell_type: Thyroid and blood\nexperimental_model: Controlled iron-deficient diets with pair-fed controls\nlimitations: Food restriction also reduced TPO; the iron effect was separately estimated. No human serum threshold or automatic iodine-treatment failure rule.\nexposure: Four weeks of graded iron restriction; separate food-restriction controls\nevidence_span: {\"source_cache\": \"artifacts/iron-research/12097675.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"26b7a5ddb0985abdb2325c7ecd505c46f02604bd4d35f68ed0c9ad67113a148e\", \"start_char\": 0, \"end_char\": 1527, \"text_sha256\": \"26b7a5ddb0985abdb2325c7ecd505c46f02604bd4d35f68ed0c9ad67113a148e\"}\n[iron-p12097675] Iron deficiency anemia reduces thyroid peroxidase activity in rats. (2002). https://pubmed.ncbi.nlm.nih.gov/12097675/ DOI: 10.1093/jn/132.7.1951","model_system":"Controlled iron-deficient diets with pair-fed controls","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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