{"id":"56b1e196-9669-5500-816a-49a1505e3e6b","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-quinolinate-nmda","predicate":"activates","statement":"Quinolinate at 75 micromolar opened NMDA channels in cultured rat hippocampal neurons in calcium-containing, magnesium-free recording solution.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d6d648cf-3e45-544c-b8c0-20f59c3f730f","mechanism_event_label":"One downstream metabolite activates an excitatory receptor.","subject":{"id":"ab444b3c-c8ec-5ada-8450-36ac9934d6b6","slug":"quinolinic-acid","display_name":"Quinolinic acid","entity_type_key":"small_molecule"},"object":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"d6d648cf-3e45-544c-b8c0-20f59c3f730f","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-quinolinate-nmda-event","event_type":"observed_relationship","label":"One downstream metabolite activates an excitatory receptor.","description":"Quinolinate at 75 micromolar opened NMDA channels in cultured rat hippocampal neurons in calcium-containing, magnesium-free recording solution.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab444b3c-c8ec-5ada-8450-36ac9934d6b6","slug":"quinolinic-acid","display_name":"Quinolinic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat CA1 neuronal single-channel recording; 1.8 mM calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Quinolinate is not tryptophan itself; bath concentration is not a dietary dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One downstream metabolite activates an excitatory receptor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Quinolinate activation of N-methyl-D-aspartate ion channels in rat hippocampal neurons. · 1990 · https://pubmed.ncbi.nlm.nih.gov/1700844/ · DOI 10.1016/0304-3940(90)90098-t","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1c410311-1876-53fd-9443-46d2a18df95b","evidence_kind":"source_excerpt","locator":"Lines 274-280","start_line":274,"end_line":280,"excerpt":"## tryptophan-quinolinate-nmda\nOne downstream metabolite activates an excitatory receptor.\nQuinolinate at 75 micromolar opened NMDA channels in cultured rat hippocampal neurons in calcium-containing, magnesium-free recording solution.\nModel: Rat CA1 neuronal single-channel recording; 1.8 mM calcium.\nLimitations: Quinolinate is not tryptophan itself; bath concentration is not a dietary dose.\nEvidence access: Primary abstract\nQuinolinate activation of N-methyl-D-aspartate ion channels in rat hippocampal neurons. · 1990 · https://pubmed.ncbi.nlm.nih.gov/1700844/ · DOI 10.1016/0304-3940(90)90098-t","model_system":"Rat CA1 neuronal single-channel recording; 1.8 mM calcium.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}