{"id":"568a1a6f-42c2-57d5-9c40-df564433bbd4","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-fldh-loss","predicate":"required_in_reductive_route","statement":"C. sporogenes fldH disruption impaired the reductive metabolism of aromatic amino acids needed for indolepropionate production.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e87dea6a-afb8-5152-bf57-b482e64c5432","mechanism_event_label":"The microbial route needs more than one enzyme.","subject":{"id":"6c5f6b75-d6ec-59e3-bcf7-7f2e2155e694","slug":"c-sporogenes-fldh","display_name":"Clostridium sporogenes FldH","entity_type_key":"protein"},"object":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"e87dea6a-afb8-5152-bf57-b482e64c5432","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-fldh-loss-event","event_type":"observed_relationship","label":"The microbial route needs more than one enzyme.","description":"C. sporogenes fldH disruption impaired the reductive metabolism of aromatic amino acids needed for indolepropionate production.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6c5f6b75-d6ec-59e3-bcf7-7f2e2155e694","slug":"c-sporogenes-fldh","display_name":"Clostridium sporogenes FldH","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text, Figure 3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Bacterial targeted-mutant metabolomics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Genetic pathway necessity is not a purified-enzyme substrate-specificity measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The microbial route needs more than one enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"30d2cdb4-8dad-5dc2-9eb7-4bf1afe07b0d","evidence_kind":"source_excerpt","locator":"Lines 498-504","start_line":498,"end_line":504,"excerpt":"## tryptophan-fldh-loss\nThe microbial route needs more than one enzyme.\nC. sporogenes fldH disruption impaired the reductive metabolism of aromatic amino acids needed for indolepropionate production.\nModel: Bacterial targeted-mutant metabolomics.\nLimitations: Genetic pathway necessity is not a purified-enzyme substrate-specificity measurement.\nEvidence access: Primary full text, Figure 3\nA gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","model_system":"Bacterial targeted-mutant metabolomics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"7dfd20063b91900cb5c6ad675e3e675b7e25ef0fb8304eabac6f263cc5518284","revision_id":"bfafd789-fdf5-521f-bd28-c662e01ec1ea","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}