{"id":"5671e160-5eca-52b5-9580-e704720b9460","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:human-mir10b-abca1","predicate":"decreases_in_recorded_experiment","statement":"Functional experiments reported that miR-10b represses ABCA1/ABCG1 in human-derived macrophages.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"4436e161-c012-5056-8d63-4c75bcf06f80","mechanism_event_label":"Functional experiments reported that miR-10b represses ABCA1/ABCG1 in human-derived macrophages.","subject":{"id":"59229f8a-819d-5903-8600-3c4b7cfb1776","slug":"human-mir-10b","display_name":"Human microRNA-10b","entity_type_key":"rna"},"object":{"id":"c5efa7cf-7e87-5809-8159-8d3f17013501","slug":"abca1","display_name":"Human ATP-binding cassette transporter A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4436e161-c012-5056-8d63-4c75bcf06f80","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:human-mir10b-abca1-event","event_type":"biochemical_relationship","label":"Functional experiments reported that miR-10b represses ABCA1/ABCG1 in human-derived macrophages.","description":"**PCA–miR-10b–cholesterol export is a specific experimental branch.** In ApoE-deficient mice, antibiotic depletion and microbiota reacquisition supported microbial involvement in C3G-to-PCA metabolism. PCA reduced macrophage miR-10b; functional tests showed miR-10b repressing ABCA1 and ABCG1 and limiting cholesterol efflux. PCA, unlike C3G at the compared concentrations, increased transporter expression and efflux. C3G feeding promoted macrophage reverse cholesterol transport and lesion regression in the microbiota-dependent mouse setting. The accessible primary abstract reports human- and mouse-derived macrophage experiments but does not fully separate every endpoint by species or list all doses; those remain extraction limits. [Wang et al., 2012](https://pubmed.ncbi.nlm.nih.gov/22821931/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"59229f8a-819d-5903-8600-3c4b7cfb1776","slug":"human-mir-10b","display_name":"Human microRNA-10b","entity_type_key":"rna"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"c5efa7cf-7e87-5809-8159-8d3f17013501","slug":"abca1","display_name":"Human ATP-binding cassette transporter A1","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"d3a1b93d-0a2c-5d1b-b672-5e4ef4b09a10","slug":"cholesterol","display_name":"Cholesterol","entity_type_key":"lipid"},"role":"exported lipid","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, figures, exact doses or endpoint-specific species assignments may remain unextracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"human-derived macrophage functional experiments; detailed intervention magnitudes and timing unextracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primary abstract identifies both species in functional analyses. Exact construct/dose/endpoint tables require full extraction. No direct PCA intervention is silently substituted for the miRNA experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Functional experiments reported that miR-10b represses ABCA1/ABCG1 in human-derived macrophages.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Gut microbiota metabolism of anthocyanin promotes reverse cholesterol transport in mice via repressing miRNA-10b. | 2012 | DOI 10.1161/circresaha.112.266502 | PMID 22821931 | https://pubmed.ncbi.nlm.nih.gov/22821931/ | https://doi.org/10.1161/circresaha.112.266502","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 66-66; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"564ee9bd-6f81-5e66-b2cc-dd9cb5961999","evidence_kind":"source_excerpt","locator":"Lines 66-66","start_line":66,"end_line":66,"excerpt":"**PCA–miR-10b–cholesterol export is a specific experimental branch.** In ApoE-deficient mice, antibiotic depletion and microbiota reacquisition supported microbial involvement in C3G-to-PCA metabolism. PCA reduced macrophage miR-10b; functional tests showed miR-10b repressing ABCA1 and ABCG1 and limiting cholesterol efflux. PCA, unlike C3G at the compared concentrations, increased transporter expression and efflux. C3G feeding promoted macrophage reverse cholesterol transport and lesion regression in the microbiota-dependent mouse setting. The accessible primary abstract reports human- and mouse-derived macrophage experiments but does not fully separate every endpoint by species or list all doses; those remain extraction limits. [Wang et al., 2012](https://pubmed.ncbi.nlm.nih.gov/22821931/).","model_system":"human-derived macrophage functional experiments; detailed intervention magnitudes and timing unextracted.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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