{"id":"567045be-d012-5be9-9829-4d681ccccb87","stable_key":"d2e91bc7-8177-5ad3-ad9b-5d65813ce082:naringenin-ampk-autophagy-dependence","predicate":"reverses","statement":"AMPK inhibition reversed naringenin-induced autophagy in LPS-stimulated macrophages, while Atg5 silencing or chloroquine counteracted its cytokine effect.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"409c9847-7237-502c-a31d-20e668d1c9ee","mechanism_event_label":"AMPK inhibition reversed naringenin-induced autophagy in LPS-stimulated macrophages, while Atg5 silencing or chloroquine counteracted its cytokine effect.","subject":{"id":"f8e1da17-6fc5-5d43-a589-a3a60e1b7137","slug":"experimental-ampk-inhibition","display_name":"Experimental AMPK inhibition","entity_type_key":"cellular_process"},"object":{"id":"80a485bd-d2ab-5ebf-9431-cf1bdafa0569","slug":"naringenin-induced-macrophage-autophagy","display_name":"Naringenin-induced macrophage autophagy","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"409c9847-7237-502c-a31d-20e668d1c9ee","stable_key":"d2e91bc7-8177-5ad3-ad9b-5d65813ce082:naringenin-ampk-autophagy-dependence-event","event_type":"observed_relationship","label":"AMPK inhibition reversed naringenin-induced autophagy in LPS-stimulated macrophages, while Atg5 silencing or chloroquine counteracted its cytokine effect.","description":"AMPK inhibition reversed naringenin-induced autophagy in LPS-stimulated macrophages, while Atg5 silencing or chloroquine counteracted its cytokine effect.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f8e1da17-6fc5-5d43-a589-a3a60e1b7137","slug":"experimental-ampk-inhibition","display_name":"Experimental AMPK inhibition","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"80a485bd-d2ab-5ebf-9431-cf1bdafa0569","slug":"naringenin-induced-macrophage-autophagy","display_name":"Naringenin-induced macrophage autophagy","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2a9350f6-01cc-5bea-8213-cd3ebb1b05cf","slug":"naringenin","display_name":"Naringenin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8128eec0-61cc-51ef-8d4e-5ec9678e8dfa","slug":"ampk-complexes","display_name":"AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"0a3986af-1635-5c8f-9460-72372d4de803","slug":"chloroquine","display_name":"Chloroquine","entity_type_key":"drug"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"c0297c97-94e4-5de9-99a2-d9c57e67b346","slug":"atg5","display_name":"Human ATG5","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"Naringenin with AMPK inhibition, Atg5 silencing or chloroquine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute cell assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. 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(2023). https://pubmed.ncbi.nlm.nih.gov/37904715/ DOI: 10.1002/iid3.983\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"LPS-stimulated RAW264.7 macrophages with a collagen-induced-arthritis mouse arm","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2804932d-46eb-5c43-8a45-0505e6b1ee05","stable_key":"import-d2e91bc7-8177-5ad3-ad9b-5d65813ce082","title":"Naringenin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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