{"id":"5660215f-606a-5d38-a5c5-43f5882f2c86","stable_key":"research:tmem189-forms-plasmenyl-pe","predicate":"desaturates","statement":"TMEM189/PEDS1 introduces the vinyl ether double bond that converts plasmanyl-PE to plasmenyl-PE.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"017d27ab-9b72-50fa-9b15-116114c7297e","mechanism_event_label":"A separate enzyme completes the characteristic plasmalogen bond.","subject":{"id":"759fe5c5-6b80-519b-8eab-2d8e42793dae","slug":"tmem189","display_name":"TMEM189","entity_type_key":"protein"},"object":{"id":"3fa1160b-a825-55ac-aa22-830517847fb0","slug":"plasmanyl-pe","display_name":"Plasmanylethanolamine","entity_type_key":"lipid"},"evidence_count":1,"mechanism_event":{"id":"017d27ab-9b72-50fa-9b15-116114c7297e","stable_key":"research:tmem189-forms-plasmenyl-pe","event_type":"desaturation","label":"A separate enzyme completes the characteristic plasmalogen bond.","description":"TMEM189/PEDS1 introduces the vinyl ether double bond that converts plasmanyl-PE to plasmenyl-PE.","status":"active","compartment":{"slug":"endoplasmic-reticulum","display_name":"Endoplasmic reticulum"},"participants":[{"entity":{"id":"759fe5c5-6b80-519b-8eab-2d8e42793dae","slug":"tmem189","display_name":"TMEM189","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3fa1160b-a825-55ac-aa22-830517847fb0","slug":"plasmanyl-pe","display_name":"Plasmanylethanolamine","entity_type_key":"lipid"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e1c84730-eb0b-5cd8-a221-dba93bc025d1","slug":"plasmenyl-pe","display_name":"Plasmenylethanolamine","entity_type_key":"lipid"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"HAP1 cells and tissues","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Knockout and rescue","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"TMEM189 is not a selenoprotein; reaction is downstream of SELENOI.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human and mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a1ce1beb-eb43-5230-852b-c63255db3b01","evidence_kind":"curated_literature_summary","locator":"lines 750-760","start_line":750,"end_line":760,"excerpt":"## tmem189-forms-plasmenyl-pe\n\nA separate enzyme completes the characteristic plasmalogen bond.\n\nTMEM189/PEDS1 introduces the vinyl ether double bond that converts plasmanyl-PE to plasmenyl-PE.\n\nOrganism: human and mouse\nCell type: HAP1 cells and tissues\nExperimental model: Knockout and rescue\nLimitations: TMEM189 is not a selenoprotein; reaction is downstream of SELENOI.\nPrimary reference: [The TMEM189 gene encodes plasmanylethanolamine desaturase which introduces the characteristic vinyl ether double bond into plasmalogens](https://pubmed.ncbi.nlm.nih.gov/32209662/)","model_system":"Knockout and rescue","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"ether-lipid-biosynthesis","plain_language":"A separate enzyme completes the characteristic plasmalogen bond.","evidence_scope":"direct_experimental","papers":[{"key":"werner-2020-tmem189","title":"The TMEM189 gene encodes plasmanylethanolamine desaturase which introduces the characteristic vinyl ether double bond into plasmalogens","url":"https://pubmed.ncbi.nlm.nih.gov/32209662/","doi":"10.1073/pnas.1917461117","year":2020,"model":"Human HAP1 knockout/rescue and mouse experiments","summary":"Identifies TMEM189/PEDS1 as the enzyme forming the plasmalogen vinyl ether bond."}]}}